Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6044 | 3' | -55.5 | NC_001813.1 | + | 28914 | 0.66 | 0.466704 |
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Target: 5'- -aCUGUCGGCACGaCGACcuCCGGCu -3' miRNA: 3'- ucGAUAGUCGUGUcGCUGucGGUCGu -5' |
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| 6044 | 3' | -55.5 | NC_001813.1 | + | 27545 | 0.69 | 0.349292 |
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Target: 5'- uGCcaUGUaGGUACAGCGAUAGCagCAGCAg -3' miRNA: 3'- uCG--AUAgUCGUGUCGCUGUCG--GUCGU- -5' |
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| 6044 | 3' | -55.5 | NC_001813.1 | + | 8473 | 0.69 | 0.33188 |
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Target: 5'- cGGCaAUgGGCugaGCGGCGACAGCUaAGCu -3' miRNA: 3'- -UCGaUAgUCG---UGUCGCUGUCGG-UCGu -5' |
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| 6044 | 3' | -55.5 | NC_001813.1 | + | 276 | 0.69 | 0.323416 |
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Target: 5'- uGCag-CAGCGCGGCGA-GGCUGGCGg -3' miRNA: 3'- uCGauaGUCGUGUCGCUgUCGGUCGU- -5' |
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| 6044 | 3' | -55.5 | NC_001813.1 | + | 13560 | 0.71 | 0.247627 |
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Target: 5'- aGGCag-CAGCuGCAGCG-CAGCCuGCAg -3' miRNA: 3'- -UCGauaGUCG-UGUCGCuGUCGGuCGU- -5' |
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| 6044 | 3' | -55.5 | NC_001813.1 | + | 28401 | 1.09 | 0.000365 |
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Target: 5'- aAGCUAUCAGCACAGCGACAGCCAGCAg -3' miRNA: 3'- -UCGAUAGUCGUGUCGCUGUCGGUCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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