Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 6056 | 5' | -51.4 | NC_001815.1 | + | 4736 | 1.08 | 0.000193 |
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Target: 5'- aAGGACCAAUGGCAUUGUUAAGGCCCUc -3' miRNA: 3'- -UCCUGGUUACCGUAACAAUUCCGGGA- -5' |
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| 6056 | 5' | -51.4 | NC_001815.1 | + | 2749 | 0.72 | 0.131918 |
|
Target: 5'- gGGaGGCCAagguggucgugAUGGCAUUGgu--GGCCCUu -3' miRNA: 3'- -UC-CUGGU-----------UACCGUAACaauuCCGGGA- -5' |
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| 6056 | 5' | -51.4 | NC_001815.1 | + | 3783 | 0.69 | 0.215154 |
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Target: 5'- aAGGACCAgAUGGUugacugccgagGUUGUgaaagcGGCCCa -3' miRNA: 3'- -UCCUGGU-UACCG-----------UAACAauu---CCGGGa -5' |
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| 6056 | 5' | -51.4 | NC_001815.1 | + | 697 | 0.67 | 0.300137 |
|
Target: 5'- aAGGACCcgaAGUGGCuggGUaccccucccggaGAGGCCCa -3' miRNA: 3'- -UCCUGG---UUACCGuaaCAa-----------UUCCGGGa -5' |
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| 6056 | 5' | -51.4 | NC_001815.1 | + | 8832 | 0.67 | 0.300137 |
|
Target: 5'- aAGGACCcgaAGUGGCuggGUaccccucccggaGAGGCCCa -3' miRNA: 3'- -UCCUGG---UUACCGuaaCAa-----------UUCCGGGa -5' |
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| 6056 | 5' | -51.4 | NC_001815.1 | + | 4834 | 0.66 | 0.349514 |
|
Target: 5'- uAGGGCUuuacuGAUGGCAUUGUcuaggggGAGGUUUg -3' miRNA: 3'- -UCCUGG-----UUACCGUAACAa------UUCCGGGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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