Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 606 | 5' | -58.3 | AC_000017.1 | + | 24707 | 0.66 | 0.370266 |
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Target: 5'- --cUGCuuGUCCGCuCGGCUGCGGUu- -3' miRNA: 3'- uaaAUGccCGGGUG-GCCGACGCCAuu -5' |
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| 606 | 5' | -58.3 | AC_000017.1 | + | 8603 | 0.66 | 0.370265 |
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Target: 5'- ---gGCGGGCCC-CCGGa---GGUAGg -3' miRNA: 3'- uaaaUGCCCGGGuGGCCgacgCCAUU- -5' |
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| 606 | 5' | -58.3 | AC_000017.1 | + | 23315 | 0.68 | 0.280448 |
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Target: 5'- ---cACGaGCCCACgGGCUGCGcGUu- -3' miRNA: 3'- uaaaUGCcCGGGUGgCCGACGC-CAuu -5' |
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| 606 | 5' | -58.3 | AC_000017.1 | + | 8532 | 0.7 | 0.208825 |
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Target: 5'- --cUACGGuaCCGCgCGGCggGCGGUGGg -3' miRNA: 3'- uaaAUGCCcgGGUG-GCCGa-CGCCAUU- -5' |
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| 606 | 5' | -58.3 | AC_000017.1 | + | 5658 | 0.7 | 0.208825 |
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Target: 5'- --cUGCGGGgCCugCGGC-GCGGa-- -3' miRNA: 3'- uaaAUGCCCgGGugGCCGaCGCCauu -5' |
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| 606 | 5' | -58.3 | AC_000017.1 | + | 10941 | 0.73 | 0.125638 |
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Target: 5'- ----uCGGGCCgGCCGGaCUGCGGcGAa -3' miRNA: 3'- uaaauGCCCGGgUGGCC-GACGCCaUU- -5' |
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| 606 | 5' | -58.3 | AC_000017.1 | + | 4623 | 0.74 | 0.111874 |
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Target: 5'- ---aAUGGGCCCACgGGCgGCGGc-- -3' miRNA: 3'- uaaaUGCCCGGGUGgCCGaCGCCauu -5' |
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| 606 | 5' | -58.3 | AC_000017.1 | + | 4979 | 1.04 | 0.000483 |
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Target: 5'- gAUUUACGGGCCCACCGGCUGCGGUAAg -3' miRNA: 3'- -UAAAUGCCCGGGUGGCCGACGCCAUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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