Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6063 | 3' | -52.7 | NC_001819.1 | + | 2357 | 0.66 | 0.295458 |
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Target: 5'- aGGAuuuUGGGUCagcaCgGAGUGuuGGGCCCCa -3' miRNA: 3'- -CCU---GCUCGGg---GgUUUACuuUCUGGGG- -5' |
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| 6063 | 3' | -52.7 | NC_001819.1 | + | 2146 | 0.67 | 0.278652 |
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Target: 5'- aGGACauuGGGCuaaagauugCCCCAAGaagccaaGAGGACCCCg -3' miRNA: 3'- -CCUG---CUCG---------GGGGUUUac-----UUUCUGGGG- -5' |
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| 6063 | 3' | -52.7 | NC_001819.1 | + | 6066 | 0.68 | 0.232804 |
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Target: 5'- gGGGCcugauacucuAGCCCCC-AGUGggGcGGCCCa -3' miRNA: 3'- -CCUGc---------UCGGGGGuUUACuuU-CUGGGg -5' |
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| 6063 | 3' | -52.7 | NC_001819.1 | + | 2307 | 0.68 | 0.218996 |
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Target: 5'- uGACGGguuGCCCCCGAcuuUGAGGGuuAUCCUg -3' miRNA: 3'- cCUGCU---CGGGGGUUu--ACUUUC--UGGGG- -5' |
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| 6063 | 3' | -52.7 | NC_001819.1 | + | 1156 | 0.69 | 0.212357 |
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Target: 5'- gGGAaguGGCCCCUAcagaAGGGGCCCCu -3' miRNA: 3'- -CCUgc-UCGGGGGUuuacUUUCUGGGG- -5' |
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| 6063 | 3' | -52.7 | NC_001819.1 | + | 8225 | 0.69 | 0.199599 |
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Target: 5'- aGGACuGGCgCCCCGAGUGAGGGGu--- -3' miRNA: 3'- -CCUGcUCG-GGGGUUUACUUUCUgggg -5' |
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| 6063 | 3' | -52.7 | NC_001819.1 | + | 5370 | 0.7 | 0.165264 |
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Target: 5'- cGcCGGGCCCCCA--UGGucucACCCCa -3' miRNA: 3'- cCuGCUCGGGGGUuuACUuuc-UGGGG- -5' |
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| 6063 | 3' | -52.7 | NC_001819.1 | + | 2572 | 0.73 | 0.108492 |
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Target: 5'- gGGAC-AGCCCCugCAAGUGcu-GACCCUa -3' miRNA: 3'- -CCUGcUCGGGG--GUUUACuuuCUGGGG- -5' |
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| 6063 | 3' | -52.7 | NC_001819.1 | + | 166 | 1.14 | 0.000056 |
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Target: 5'- cGGACGAGCCCCCAAAUGAAAGACCCCc -3' miRNA: 3'- -CCUGCUCGGGGGUUUACUUUCUGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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