Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 608 | 3' | -53 | AC_000017.1 | + | 13992 | 0.66 | 0.748838 |
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Target: 5'- cGACAGCagcguccugGAUuuGGGAGGGAGUGGc- -3' miRNA: 3'- uCUGUCG---------CUAcgCCUUCUCUCACUcc -5' |
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| 608 | 3' | -53 | AC_000017.1 | + | 10354 | 0.66 | 0.693225 |
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Target: 5'- cGGCGGCGGcugGCGGuAGAGGGgccagcguAGGg -3' miRNA: 3'- uCUGUCGCUa--CGCCuUCUCUCac------UCC- -5' |
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| 608 | 3' | -53 | AC_000017.1 | + | 955 | 0.71 | 0.407849 |
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Target: 5'- uGACGaCGAgGauGAAGAGGGUGAGGa -3' miRNA: 3'- uCUGUcGCUaCgcCUUCUCUCACUCC- -5' |
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| 608 | 3' | -53 | AC_000017.1 | + | 15089 | 0.72 | 0.388732 |
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Target: 5'- uGGCAGCGgcGCGGAAGAGAa----- -3' miRNA: 3'- uCUGUCGCuaCGCCUUCUCUcacucc -5' |
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| 608 | 3' | -53 | AC_000017.1 | + | 9407 | 0.74 | 0.286941 |
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Target: 5'- uGGCGGCGGUGgGGGAGGGGG-GAc- -3' miRNA: 3'- uCUGUCGCUACgCCUUCUCUCaCUcc -5' |
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| 608 | 3' | -53 | AC_000017.1 | + | 6111 | 1.11 | 0.000691 |
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Target: 5'- cAGACAGCGAUGCGGAAGAGAGUGAGGa -3' miRNA: 3'- -UCUGUCGCUACGCCUUCUCUCACUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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