Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
608 | 5' | -58.1 | AC_000017.1 | + | 5969 | 0.66 | 0.476222 |
Target: 5'- -aCCCUggagcgaguggACCCCCUagUGgacaacgaccgcUACCCCuCCCa -3' miRNA: 3'- cgGGGG-----------UGGGGGAaaAU------------AUGGGG-GGG- -5' |
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608 | 5' | -58.1 | AC_000017.1 | + | 24118 | 0.66 | 0.476222 |
Target: 5'- aGCCUaaCCGCCCCCUcugaGUucgccaccACCgCCUCCa -3' miRNA: 3'- -CGGG--GGUGGGGGAaaa-UA--------UGG-GGGGG- -5' |
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608 | 5' | -58.1 | AC_000017.1 | + | 6756 | 0.66 | 0.467089 |
Target: 5'- cGCUcaCCCACCCCaugccauggGgUCCCCCa -3' miRNA: 3'- -CGG--GGGUGGGGgaaaaua--UgGGGGGG- -5' |
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608 | 5' | -58.1 | AC_000017.1 | + | 35879 | 0.67 | 0.398472 |
Target: 5'- cGUUCCCACgCCCCgcgccacGUcacaaacuccACCCCCUCa -3' miRNA: 3'- -CGGGGGUG-GGGGaaaa---UA----------UGGGGGGG- -5' |
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608 | 5' | -58.1 | AC_000017.1 | + | 123 | 0.67 | 0.398472 |
Target: 5'- cGUUCCCACgCCCCgcgccacGUcacaaacuccACCCCCUCa -3' miRNA: 3'- -CGGGGGUG-GGGGaaaa---UA----------UGGGGGGG- -5' |
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608 | 5' | -58.1 | AC_000017.1 | + | 27449 | 0.67 | 0.389337 |
Target: 5'- cGCUCCCACCa-CUgugGUACUUCCCa -3' miRNA: 3'- -CGGGGGUGGggGAaaaUAUGGGGGGg -5' |
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608 | 5' | -58.1 | AC_000017.1 | + | 19353 | 0.67 | 0.371482 |
Target: 5'- uGCCUCCuCCUCCUc---UGCCUCCUCc -3' miRNA: 3'- -CGGGGGuGGGGGAaaauAUGGGGGGG- -5' |
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608 | 5' | -58.1 | AC_000017.1 | + | 24487 | 0.68 | 0.362767 |
Target: 5'- cGCCaCCUguuCUCaCCgcgUGUACCCCCCa -3' miRNA: 3'- -CGG-GGGu--GGG-GGaaaAUAUGGGGGGg -5' |
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608 | 5' | -58.1 | AC_000017.1 | + | 24378 | 0.68 | 0.362767 |
Target: 5'- cCCCCCGCCCgacuugcuCCUUguuUGCCUCUgCg -3' miRNA: 3'- cGGGGGUGGG--------GGAAaauAUGGGGGgG- -5' |
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608 | 5' | -58.1 | AC_000017.1 | + | 4912 | 0.68 | 0.357606 |
Target: 5'- uCCCCUACCCCggaaaccguuuucUUUAUugCCCCgCa -3' miRNA: 3'- cGGGGGUGGGGga-----------AAAUAugGGGGgG- -5' |
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608 | 5' | -58.1 | AC_000017.1 | + | 18326 | 0.68 | 0.354194 |
Target: 5'- aUCCCCGCCCUCccgUAgaggaGCCUCCaCCg -3' miRNA: 3'- cGGGGGUGGGGGaaaAUa----UGGGGG-GG- -5' |
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608 | 5' | -58.1 | AC_000017.1 | + | 30127 | 0.68 | 0.329344 |
Target: 5'- uGCUCCCagACCUCCaa----GCCUCCCCa -3' miRNA: 3'- -CGGGGG--UGGGGGaaaauaUGGGGGGG- -5' |
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608 | 5' | -58.1 | AC_000017.1 | + | 14229 | 0.69 | 0.321351 |
Target: 5'- gGCgCUgGguuCCCCCUUcgAUGCUCCCCUg -3' miRNA: 3'- -CGgGGgU---GGGGGAAaaUAUGGGGGGG- -5' |
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608 | 5' | -58.1 | AC_000017.1 | + | 23998 | 0.7 | 0.269479 |
Target: 5'- cGCCgCCGCCUCCccgc--GCgCCCCCa -3' miRNA: 3'- -CGGgGGUGGGGGaaaauaUGgGGGGG- -5' |
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608 | 5' | -58.1 | AC_000017.1 | + | 31151 | 0.7 | 0.262645 |
Target: 5'- uUCCUUACCCCUcccUUUGU-CUCCCCCa -3' miRNA: 3'- cGGGGGUGGGGGa--AAAUAuGGGGGGG- -5' |
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608 | 5' | -58.1 | AC_000017.1 | + | 17451 | 0.7 | 0.242354 |
Target: 5'- cGCgCCUACCCCCggcUAucguggcuacaccUACCgCCCCa -3' miRNA: 3'- -CGgGGGUGGGGGaaaAU-------------AUGGgGGGG- -5' |
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608 | 5' | -58.1 | AC_000017.1 | + | 27628 | 0.71 | 0.239206 |
Target: 5'- -aCCUCGCCCUCUgauuuucaggugagUUAUACCCUgCCCg -3' miRNA: 3'- cgGGGGUGGGGGAa-------------AAUAUGGGG-GGG- -5' |
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608 | 5' | -58.1 | AC_000017.1 | + | 12997 | 0.73 | 0.17157 |
Target: 5'- aGCCCgCACCCCCcaca--GCCCCUgCa -3' miRNA: 3'- -CGGGgGUGGGGGaaaauaUGGGGGgG- -5' |
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608 | 5' | -58.1 | AC_000017.1 | + | 10434 | 0.75 | 0.109772 |
Target: 5'- aGCCCCgGCCaCCCUacgcugGCCCCUCUa -3' miRNA: 3'- -CGGGGgUGG-GGGAaaaua-UGGGGGGG- -5' |
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608 | 5' | -58.1 | AC_000017.1 | + | 2391 | 0.79 | 0.058199 |
Target: 5'- cCCCCCGCUCCCUcuu-UACCCCCUUu -3' miRNA: 3'- cGGGGGUGGGGGAaaauAUGGGGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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