miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
6081 5' -43.2 NC_001819.1 + 3984 0.66 0.877647
Target:  5'- gCCUGguagugggucauucGGGCGUu--GGAGAGCCAGc -3'
miRNA:   3'- aGGAUa-------------UCUGUAuuuUCUCUUGGUCa -5'
6081 5' -43.2 NC_001819.1 + 1340 0.67 0.795141
Target:  5'- uUCCaguUAUAGAgGUcAGAGGAGGaaaaugGCCAGUa -3'
miRNA:   3'- -AGG---AUAUCUgUA-UUUUCUCU------UGGUCA- -5'
6081 5' -43.2 NC_001819.1 + 4486 0.68 0.782653
Target:  5'- gUCUUuUGGGCA-GGAAGAGAGCCuucAGUa -3'
miRNA:   3'- -AGGAuAUCUGUaUUUUCUCUUGG---UCA- -5'
6081 5' -43.2 NC_001819.1 + 7182 0.68 0.756942
Target:  5'- -gCUAUAGACGUAAcuGGGAGGAUggUAGg -3'
miRNA:   3'- agGAUAUCUGUAUU--UUCUCUUG--GUCa -5'
6081 5' -43.2 NC_001819.1 + 7186 1.11 0.001196
Target:  5'- aUCCUAUAGACAUAAAAGAGAACCAGUg -3'
miRNA:   3'- -AGGAUAUCUGUAUUUUCUCUUGGUCA- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.