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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6086 | 5' | -48.7 | NC_001822.1 | + | 1930 | 1.12 | 0.000048 |
Target: 5'- cCAUCAUUAAGCACUCUACCACCCUCCc -3' miRNA: 3'- -GUAGUAAUUCGUGAGAUGGUGGGAGG- -5' |
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6086 | 5' | -48.7 | NC_001822.1 | + | 862 | 0.69 | 0.16133 |
Target: 5'- --cCAUgaaaacuCGCUCUACCAggcCCCUCCg -3' miRNA: 3'- guaGUAauuc---GUGAGAUGGU---GGGAGG- -5' |
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6086 | 5' | -48.7 | NC_001822.1 | + | 3161 | 0.66 | 0.253075 |
Target: 5'- --gCAUUGcuguGCAUgcgUCUACUGCCCUCa -3' miRNA: 3'- guaGUAAUu---CGUG---AGAUGGUGGGAGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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