Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 609 | 3' | -57.9 | AC_000017.1 | + | 11164 | 0.66 | 0.446914 |
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Target: 5'- cGCGGcuGACGCGGCggcAGAuggugauuacgaaCCCCCgcggcGCCGg -3' miRNA: 3'- -UGCU--CUGCGUCGa--UCU-------------GGGGGa----UGGC- -5' |
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| 609 | 3' | -57.9 | AC_000017.1 | + | 24751 | 0.66 | 0.438028 |
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Target: 5'- aGCGAGGCGauauCAGgUAuGACagcgCCCUGCCGc -3' miRNA: 3'- -UGCUCUGC----GUCgAU-CUGg---GGGAUGGC- -5' |
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| 609 | 3' | -57.9 | AC_000017.1 | + | 8715 | 0.67 | 0.372566 |
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Target: 5'- cGCGAcGACGCGGCgguuGAUCUCCUGaauCUGg -3' miRNA: 3'- -UGCU-CUGCGUCGau--CUGGGGGAU---GGC- -5' |
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| 609 | 3' | -57.9 | AC_000017.1 | + | 9663 | 0.67 | 0.372566 |
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Target: 5'- uUGAGAUGCAucGUUAGcGCCguauCCCUGCCGc -3' miRNA: 3'- uGCUCUGCGU--CGAUC-UGG----GGGAUGGC- -5' |
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| 609 | 3' | -57.9 | AC_000017.1 | + | 24435 | 0.69 | 0.276733 |
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Target: 5'- -aGAG-CGCAGCgauGugCCCCUcGCCa -3' miRNA: 3'- ugCUCuGCGUCGau-CugGGGGA-UGGc -5' |
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| 609 | 3' | -57.9 | AC_000017.1 | + | 11240 | 0.7 | 0.256074 |
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Target: 5'- gGCGAGggccugGCGCGGCUAGgagcGCCCUCUcCUGa -3' miRNA: 3'- -UGCUC------UGCGUCGAUC----UGGGGGAuGGC- -5' |
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| 609 | 3' | -57.9 | AC_000017.1 | + | 26917 | 0.7 | 0.243015 |
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Target: 5'- gGCGccAGACGCAGCgcuccuCCUCCUGCUGc -3' miRNA: 3'- -UGC--UCUGCGUCGaucu--GGGGGAUGGC- -5' |
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| 609 | 3' | -57.9 | AC_000017.1 | + | 12133 | 0.72 | 0.196242 |
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Target: 5'- gACGcGGGCGCugaccugcGCUGGGCCCCaaGCCGa -3' miRNA: 3'- -UGC-UCUGCGu-------CGAUCUGGGGgaUGGC- -5' |
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| 609 | 3' | -57.9 | AC_000017.1 | + | 6595 | 1.07 | 0.000411 |
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Target: 5'- gACGAGACGCAGCUAGACCCCCUACCGc -3' miRNA: 3'- -UGCUCUGCGUCGAUCUGGGGGAUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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