Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
609 | 5' | -63 | AC_000017.1 | + | 16276 | 0.66 | 0.247957 |
Target: 5'- -cCGCGCGuuGGGCGGCaguGCCgggUCgGCg -3' miRNA: 3'- gaGCGCGUu-CCCGCCGg--CGG---AGaCGa -5' |
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609 | 5' | -63 | AC_000017.1 | + | 33242 | 0.67 | 0.20084 |
Target: 5'- aUCGUGCAucaggauAGGGCGGUgGUg-CUGCa -3' miRNA: 3'- gAGCGCGU-------UCCCGCCGgCGgaGACGa -5' |
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609 | 5' | -63 | AC_000017.1 | + | 23943 | 0.68 | 0.153996 |
Target: 5'- -gCGCGCgGGGGGCGGCgGCgaCgGCg -3' miRNA: 3'- gaGCGCG-UUCCCGCCGgCGgaGaCGa -5' |
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609 | 5' | -63 | AC_000017.1 | + | 16234 | 0.69 | 0.149858 |
Target: 5'- -aCGCGC---GGCGGCgGCC-CUGCUu -3' miRNA: 3'- gaGCGCGuucCCGCCGgCGGaGACGA- -5' |
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609 | 5' | -63 | AC_000017.1 | + | 8567 | 0.7 | 0.120244 |
Target: 5'- gUCGCGCcgcGGGGauGCgGCCUCUuGCa -3' miRNA: 3'- gAGCGCGu--UCCCgcCGgCGGAGA-CGa -5' |
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609 | 5' | -63 | AC_000017.1 | + | 11488 | 0.7 | 0.116949 |
Target: 5'- -gCGCGCAcacGuGGCGGCCGCCgacCUGgUa -3' miRNA: 3'- gaGCGCGUu--C-CCGCCGGCGGa--GACgA- -5' |
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609 | 5' | -63 | AC_000017.1 | + | 15284 | 0.71 | 0.09889 |
Target: 5'- -cCGCaGCGGGGGCGGCaGCUUCggccGCUg -3' miRNA: 3'- gaGCG-CGUUCCCGCCGgCGGAGa---CGA- -5' |
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609 | 5' | -63 | AC_000017.1 | + | 6488 | 0.71 | 0.095878 |
Target: 5'- gCUCGUugguccaGCAGaGGCGGCCGCCcUUGCg -3' miRNA: 3'- -GAGCG-------CGUUcCCGCCGGCGGaGACGa -5' |
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609 | 5' | -63 | AC_000017.1 | + | 20879 | 0.72 | 0.07667 |
Target: 5'- cCUCGCGCAAcuGGGCGGCUuuccgaggcuggGCCUUUacGCg -3' miRNA: 3'- -GAGCGCGUU--CCCGCCGG------------CGGAGA--CGa -5' |
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609 | 5' | -63 | AC_000017.1 | + | 22733 | 0.73 | 0.068407 |
Target: 5'- aCUCGCGCGcgcAGGGCGGagGCCccaaCUGCg -3' miRNA: 3'- -GAGCGCGU---UCCCGCCggCGGa---GACGa -5' |
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609 | 5' | -63 | AC_000017.1 | + | 22103 | 0.76 | 0.039452 |
Target: 5'- -gCGUGCAGGGGCccaacucGGCCGCCuguggccuauUCUGCUg -3' miRNA: 3'- gaGCGCGUUCCCG-------CCGGCGG----------AGACGA- -5' |
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609 | 5' | -63 | AC_000017.1 | + | 6561 | 1.07 | 0.000122 |
Target: 5'- gCUCGCGCAAGGGCGGCCGCCUCUGCUg -3' miRNA: 3'- -GAGCGCGUUCCCGCCGGCGGAGACGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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