Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6092 | 3' | -64.3 | NC_001824.1 | + | 29758 | 0.68 | 0.34375 |
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Target: 5'- uGGGCCu---GGgAGCCCgGGGCUgCCu -3' miRNA: 3'- uCCUGGugguCCgUCGGGgCCCGA-GG- -5' |
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| 6092 | 3' | -64.3 | NC_001824.1 | + | 29352 | 0.68 | 0.314723 |
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Target: 5'- uGGGACCACUGGG--GCCa-GGGcCUCCa -3' miRNA: 3'- -UCCUGGUGGUCCguCGGggCCC-GAGG- -5' |
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| 6092 | 3' | -64.3 | NC_001824.1 | + | 29633 | 0.71 | 0.212107 |
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Target: 5'- cAGGACCACaAGGCGaCaaUGGGCUCCc -3' miRNA: 3'- -UCCUGGUGgUCCGUcGggGCCCGAGG- -5' |
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| 6092 | 3' | -64.3 | NC_001824.1 | + | 29821 | 0.81 | 0.040111 |
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Target: 5'- cAGGAUCACUAGGCAGgCCCGGGg-CCu -3' miRNA: 3'- -UCCUGGUGGUCCGUCgGGGCCCgaGG- -5' |
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| 6092 | 3' | -64.3 | NC_001824.1 | + | 29750 | 0.9 | 0.008238 |
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Target: 5'- uAGGACCACCAGGCGcCCCCGGGCcCCc -3' miRNA: 3'- -UCCUGGUGGUCCGUcGGGGCCCGaGG- -5' |
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| 6092 | 3' | -64.3 | NC_001824.1 | + | 29893 | 1.09 | 0.000295 |
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Target: 5'- cAGGACCACCAGGCAGCCCCGGGCUCCc -3' miRNA: 3'- -UCCUGGUGGUCCGUCGGGGCCCGAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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