Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6093 | 3' | -53.1 | NC_001824.1 | + | 29525 | 0.67 | 0.935222 |
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Target: 5'- -aAGGCGCUGaUGgacAACCGGguACCCc -3' miRNA: 3'- ggUCCGCGGCaACaguUUGGCU--UGGG- -5' |
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| 6093 | 3' | -53.1 | NC_001824.1 | + | 64561 | 0.67 | 0.918821 |
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Target: 5'- gUAGGUGCUuauGUUGUUGAACCaguACCUg -3' miRNA: 3'- gGUCCGCGG---CAACAGUUUGGcu-UGGG- -5' |
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| 6093 | 3' | -53.1 | NC_001824.1 | + | 29743 | 0.68 | 0.879248 |
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Target: 5'- aCCAGGCGCCc--------CCGGGCCCc -3' miRNA: 3'- -GGUCCGCGGcaacaguuuGGCUUGGG- -5' |
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| 6093 | 3' | -53.1 | NC_001824.1 | + | 80340 | 1.1 | 0.00383 |
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Target: 5'- gCCAGGCGCCGUUGUCAAACCGAACCUu -3' miRNA: 3'- -GGUCCGCGGCAACAGUUUGGCUUGGG- -5' |
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| 6093 | 3' | -53.1 | NC_001824.1 | + | 80376 | 1.13 | 0.002222 |
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Target: 5'- gCCAGGCGCCGUUGUCAAACCGAACCCc -3' miRNA: 3'- -GGUCCGCGGCAACAGUUUGGCUUGGG- -5' |
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| 6093 | 3' | -53.1 | NC_001824.1 | + | 80412 | 1.13 | 0.002222 |
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Target: 5'- gCCAGGCGCCGUUGUCAAACCGAACCCc -3' miRNA: 3'- -GGUCCGCGGCAACAGUUUGGCUUGGG- -5' |
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| 6093 | 3' | -53.1 | NC_001824.1 | + | 80448 | 1.13 | 0.002222 |
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Target: 5'- gCCAGGCGCCGUUGUCAAACCGAACCCc -3' miRNA: 3'- -GGUCCGCGGCAACAGUUUGGCUUGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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