Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6096 | 3' | -43.9 | NC_001824.1 | + | 29667 | 0.66 | 0.999987 |
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Target: 5'- ---gGCAA--GCCAGGUaauCCUGGc -3' miRNA: 3'- uuaaUGUUaaUGGUCCAaauGGACCa -5' |
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| 6096 | 3' | -43.9 | NC_001824.1 | + | 29763 | 0.66 | 0.999982 |
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Target: 5'- --cUGgGAgc-CCGGGgcUGCCUGGUg -3' miRNA: 3'- uuaAUgUUaauGGUCCaaAUGGACCA- -5' |
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| 6096 | 3' | -43.9 | NC_001824.1 | + | 29372 | 0.66 | 0.999964 |
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Target: 5'- ---------aACCAGGUaaACCUGGUa -3' miRNA: 3'- uuaauguuaaUGGUCCAaaUGGACCA- -5' |
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| 6096 | 3' | -43.9 | NC_001824.1 | + | 29415 | 0.69 | 0.999077 |
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Target: 5'- ---aGCGAgauuccuggACCGacuGGUUUACCUGGUa -3' miRNA: 3'- uuaaUGUUaa-------UGGU---CCAAAUGGACCA- -5' |
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| 6096 | 3' | -43.9 | NC_001824.1 | + | 29540 | 0.73 | 0.985054 |
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Target: 5'- ---gGCAA--GCCAGGaUUACCUGGc -3' miRNA: 3'- uuaaUGUUaaUGGUCCaAAUGGACCa -5' |
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| 6096 | 3' | -43.9 | NC_001824.1 | + | 29243 | 1.06 | 0.041126 |
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Target: 5'- cAAUUACAAUUACCAGGUUUACCUGGUu -3' miRNA: 3'- -UUAAUGUUAAUGGUCCAAAUGGACCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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