Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6096 | 5' | -52.2 | NC_001824.1 | + | 29897 | 0.66 | 0.960409 |
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Target: 5'- cUGCCAGGaccACCAGgcagcccCGGgcucCCAGGc -3' miRNA: 3'- aAUGGUCCauuUGGUCa------GCCa---GGUCC- -5' |
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| 6096 | 5' | -52.2 | NC_001824.1 | + | 29825 | 0.67 | 0.933259 |
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Target: 5'- cUGCCAGGaucACUAGgcaGGcCCGGGg -3' miRNA: 3'- aAUGGUCCauuUGGUCag-CCaGGUCC- -5' |
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| 6096 | 5' | -52.2 | NC_001824.1 | + | 29331 | 0.67 | 0.927814 |
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Target: 5'- -cGCUAGGUccuguaGGGCCAGUgGGaccacuggggCCAGGg -3' miRNA: 3'- aaUGGUCCA------UUUGGUCAgCCa---------GGUCC- -5' |
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| 6096 | 5' | -52.2 | NC_001824.1 | + | 29560 | 0.67 | 0.927814 |
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Target: 5'- cUUGCCAGGUGGGCCA--UGGaaaCCAGc -3' miRNA: 3'- -AAUGGUCCAUUUGGUcaGCCa--GGUCc -5' |
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| 6096 | 5' | -52.2 | NC_001824.1 | + | 29392 | 0.69 | 0.833634 |
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Target: 5'- uUUACCuGGUAAACCAGguaaaCCAGGu -3' miRNA: 3'- -AAUGGuCCAUUUGGUCagccaGGUCC- -5' |
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| 6096 | 5' | -52.2 | NC_001824.1 | + | 29931 | 0.74 | 0.587562 |
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Target: 5'- -cACCAGGU-GACCuGUUcGUCCAGGu -3' miRNA: 3'- aaUGGUCCAuUUGGuCAGcCAGGUCC- -5' |
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| 6096 | 5' | -52.2 | NC_001824.1 | + | 29278 | 1.09 | 0.004071 |
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Target: 5'- uUUACCAGGUAAACCAGUCGGUCCAGGa -3' miRNA: 3'- -AAUGGUCCAUUUGGUCAGCCAGGUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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