Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6098 | 5' | -44 | NC_001824.1 | + | 101043 | 0.66 | 0.99999 |
Target: 5'- aUACUGaUAuACCuauACCAACGuacgaguguuggaucAGAGCGUg -3' miRNA: 3'- -AUGACaAUuUGG---UGGUUGU---------------UUUCGCG- -5' |
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6098 | 5' | -44 | NC_001824.1 | + | 46068 | 0.66 | 0.999988 |
Target: 5'- aACUGUUAAACCAauuaaaaacaugUCAGUAAGuaccauggauGGCGCu -3' miRNA: 3'- aUGACAAUUUGGU------------GGUUGUUU----------UCGCG- -5' |
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6098 | 5' | -44 | NC_001824.1 | + | 29762 | 0.66 | 0.999983 |
Target: 5'- aUACUGgccugcuaGGACCACCA-----GGCGCc -3' miRNA: 3'- -AUGACaa------UUUGGUGGUuguuuUCGCG- -5' |
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6098 | 5' | -44 | NC_001824.1 | + | 21996 | 0.67 | 0.999954 |
Target: 5'- aUACUGcaAAGCCGCCuaaAACAGGAcCGUa -3' miRNA: 3'- -AUGACaaUUUGGUGG---UUGUUUUcGCG- -5' |
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6098 | 5' | -44 | NC_001824.1 | + | 38034 | 0.67 | 0.999938 |
Target: 5'- -cCUGccaUUGAACC-CCAACcuaauGGCGCa -3' miRNA: 3'- auGAC---AAUUUGGuGGUUGuuu--UCGCG- -5' |
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6098 | 5' | -44 | NC_001824.1 | + | 97279 | 0.67 | 0.999916 |
Target: 5'- -uCUGUUAAAUCuguucuuguACCAACA--AGCGUc -3' miRNA: 3'- auGACAAUUUGG---------UGGUUGUuuUCGCG- -5' |
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6098 | 5' | -44 | NC_001824.1 | + | 97468 | 0.67 | 0.999916 |
Target: 5'- cACUGUucuuuauuuUGAuCCGCUAACGAuuuaacAAGCGUa -3' miRNA: 3'- aUGACA---------AUUuGGUGGUUGUU------UUCGCG- -5' |
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6098 | 5' | -44 | NC_001824.1 | + | 79083 | 0.67 | 0.999916 |
Target: 5'- aUGCUGUU--GCCACCGAUcugccAAGUGg -3' miRNA: 3'- -AUGACAAuuUGGUGGUUGuu---UUCGCg -5' |
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6098 | 5' | -44 | NC_001824.1 | + | 57376 | 0.67 | 0.999851 |
Target: 5'- -uCUGg--GGCCACUuACAAAcGGCGCa -3' miRNA: 3'- auGACaauUUGGUGGuUGUUU-UCGCG- -5' |
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6098 | 5' | -44 | NC_001824.1 | + | 85401 | 0.68 | 0.999804 |
Target: 5'- cUACUGUUuugauaacgaAGGCUuuauuucuugGCCGGCGcuuAAAGCGCa -3' miRNA: 3'- -AUGACAA----------UUUGG----------UGGUUGU---UUUCGCG- -5' |
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6098 | 5' | -44 | NC_001824.1 | + | 34817 | 1.12 | 0.019286 |
Target: 5'- uUACUGUUAAACCACCAACAAAAGCGCc -3' miRNA: 3'- -AUGACAAUUUGGUGGUUGUUUUCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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