Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
610 | 3' | -56.5 | AC_000017.1 | + | 14000 | 0.66 | 0.536676 |
Target: 5'- aGACCCcgcugacggucGUGCcuuugacgacggguGGGCGgGCGCggGCCGg -3' miRNA: 3'- gUUGGG-----------UAUG--------------CUCGCgCGCGaaCGGC- -5' |
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610 | 3' | -56.5 | AC_000017.1 | + | 12962 | 0.66 | 0.529036 |
Target: 5'- --uCCCAcagGCGAcGCGCGaccgugucuaGCUUGCUGa -3' miRNA: 3'- guuGGGUa--UGCU-CGCGCg---------CGAACGGC- -5' |
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610 | 3' | -56.5 | AC_000017.1 | + | 17862 | 0.66 | 0.496777 |
Target: 5'- -cGCgCAUGCGAcgguGCGaCGCGCgccGCCGc -3' miRNA: 3'- guUGgGUAUGCU----CGC-GCGCGaa-CGGC- -5' |
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610 | 3' | -56.5 | AC_000017.1 | + | 29824 | 0.67 | 0.47577 |
Target: 5'- uGACCCuuauUGCGcuuuucuGCGCGUGCUccacauugGCCGc -3' miRNA: 3'- gUUGGGu---AUGCu------CGCGCGCGAa-------CGGC- -5' |
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610 | 3' | -56.5 | AC_000017.1 | + | 18098 | 0.67 | 0.465433 |
Target: 5'- --uCCCAUgaACGGGCGCGaGCcgUGUCGc -3' miRNA: 3'- guuGGGUA--UGCUCGCGCgCGa-ACGGC- -5' |
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610 | 3' | -56.5 | AC_000017.1 | + | 8742 | 0.67 | 0.432183 |
Target: 5'- gCAACCUccGUGCGcAGCaccagcuccugcccGCGCGCggcGCCGa -3' miRNA: 3'- -GUUGGG--UAUGC-UCG--------------CGCGCGaa-CGGC- -5' |
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610 | 3' | -56.5 | AC_000017.1 | + | 15183 | 0.68 | 0.415604 |
Target: 5'- ---gCCAcACGGGCggaggagaaGCGCGCUgagGCCGa -3' miRNA: 3'- guugGGUaUGCUCG---------CGCGCGAa--CGGC- -5' |
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610 | 3' | -56.5 | AC_000017.1 | + | 16314 | 0.69 | 0.359456 |
Target: 5'- uCGGCCgGUGCGAcGUGCGCGgUUaagcaggGCCGc -3' miRNA: 3'- -GUUGGgUAUGCU-CGCGCGCgAA-------CGGC- -5' |
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610 | 3' | -56.5 | AC_000017.1 | + | 23312 | 0.69 | 0.334684 |
Target: 5'- gAGCCC--ACGGGCuGCGCGUUgugGCUGc -3' miRNA: 3'- gUUGGGuaUGCUCG-CGCGCGAa--CGGC- -5' |
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610 | 3' | -56.5 | AC_000017.1 | + | 12276 | 0.7 | 0.310397 |
Target: 5'- -cGCCgCcgACGuugccAGCGCGCGCgggUGCCa -3' miRNA: 3'- guUGG-GuaUGC-----UCGCGCGCGa--ACGGc -5' |
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610 | 3' | -56.5 | AC_000017.1 | + | 31306 | 0.7 | 0.302605 |
Target: 5'- -uGCCCAUuuUGAGCGCaaGCaUGCCGu -3' miRNA: 3'- guUGGGUAu-GCUCGCGcgCGaACGGC- -5' |
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610 | 3' | -56.5 | AC_000017.1 | + | 3723 | 0.71 | 0.265906 |
Target: 5'- cCGGCCCAUGgGGGCaUGCGCgUUGUCa -3' miRNA: 3'- -GUUGGGUAUgCUCGcGCGCG-AACGGc -5' |
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610 | 3' | -56.5 | AC_000017.1 | + | 5475 | 0.72 | 0.239209 |
Target: 5'- cCAGCCCcuccGCGgcauggcccuuGGCGCGCaGCUUGCCc -3' miRNA: 3'- -GUUGGGua--UGC-----------UCGCGCG-CGAACGGc -5' |
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610 | 3' | -56.5 | AC_000017.1 | + | 12332 | 0.72 | 0.220686 |
Target: 5'- gGACCCggcgGUGCGGGCG-GCGCUgcagaGCCa -3' miRNA: 3'- gUUGGG----UAUGCUCGCgCGCGAa----CGGc -5' |
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610 | 3' | -56.5 | AC_000017.1 | + | 6723 | 1.07 | 0.000554 |
Target: 5'- uCAACCCAUACGAGCGCGCGCUUGCCGc -3' miRNA: 3'- -GUUGGGUAUGCUCGCGCGCGAACGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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