Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6103 | 3' | -52.6 | NC_001826.1 | + | 68770 | 0.66 | 0.961028 |
Target: 5'- --cUGUGGCgGaagaagGGCCAGGgcuaaugggugaaaaUGGCCCc -3' miRNA: 3'- cauACAUUGgUa-----CCGGUCCa--------------ACCGGG- -5' |
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6103 | 3' | -52.6 | NC_001826.1 | + | 4931 | 0.66 | 0.952742 |
Target: 5'- --cUGggGCCuUGGCCAGGguaGCUCa -3' miRNA: 3'- cauACauUGGuACCGGUCCaacCGGG- -5' |
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6103 | 3' | -52.6 | NC_001826.1 | + | 86863 | 0.66 | 0.948408 |
Target: 5'- ---cGUGGuCCAgcugggcauauUGGCCAGGagauaagaGGCCCa -3' miRNA: 3'- cauaCAUU-GGU-----------ACCGGUCCaa------CCGGG- -5' |
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6103 | 3' | -52.6 | NC_001826.1 | + | 107520 | 0.66 | 0.945686 |
Target: 5'- -aGUGgGACCcgGGCguGGUccaguauccugggucUGGCCg -3' miRNA: 3'- caUACaUUGGuaCCGguCCA---------------ACCGGg -5' |
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6103 | 3' | -52.6 | NC_001826.1 | + | 113065 | 0.66 | 0.94382 |
Target: 5'- --cUGUAAUUGUGGUguGGUggcaggUGGCCa -3' miRNA: 3'- cauACAUUGGUACCGguCCA------ACCGGg -5' |
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6103 | 3' | -52.6 | NC_001826.1 | + | 101579 | 0.66 | 0.94382 |
Target: 5'- gGUGUGgauaguUGGCaauaGGCCAGGUgGGCCg -3' miRNA: 3'- -CAUAC------AUUGgua-CCGGUCCAaCCGGg -5' |
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6103 | 3' | -52.6 | NC_001826.1 | + | 64814 | 0.68 | 0.90439 |
Target: 5'- -cAUGggGGCCA-GGCCAauGGUUuGGCCUu -3' miRNA: 3'- caUACa-UUGGUaCCGGU--CCAA-CCGGG- -5' |
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6103 | 3' | -52.6 | NC_001826.1 | + | 79986 | 0.68 | 0.890788 |
Target: 5'- gGUAUGUAGCCAgucaaguuUGGCUuucaAGGUagGGUCa -3' miRNA: 3'- -CAUACAUUGGU--------ACCGG----UCCAa-CCGGg -5' |
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6103 | 3' | -52.6 | NC_001826.1 | + | 72721 | 0.69 | 0.844163 |
Target: 5'- ---gGUGACUcacuugggggcGUGGCCaaAGGUUGGCUa -3' miRNA: 3'- cauaCAUUGG-----------UACCGG--UCCAACCGGg -5' |
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6103 | 3' | -52.6 | NC_001826.1 | + | 118556 | 0.71 | 0.750592 |
Target: 5'- ---gGgcGCCggGGCCGGaGcUGGCCCu -3' miRNA: 3'- cauaCauUGGuaCCGGUC-CaACCGGG- -5' |
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6103 | 3' | -52.6 | NC_001826.1 | + | 2217 | 0.72 | 0.730174 |
Target: 5'- -aAUGuUGACCAcagaUGGCCAGuGagcuaugcuUUGGCCCa -3' miRNA: 3'- caUAC-AUUGGU----ACCGGUC-C---------AACCGGG- -5' |
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6103 | 3' | -52.6 | NC_001826.1 | + | 10583 | 0.72 | 0.713567 |
Target: 5'- -gGUGUccACCcgGGUCccucacagacugcguGGGUUGGCCCa -3' miRNA: 3'- caUACAu-UGGuaCCGG---------------UCCAACCGGG- -5' |
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6103 | 3' | -52.6 | NC_001826.1 | + | 47155 | 0.76 | 0.49822 |
Target: 5'- -gGUGUGACacugcgGGCCAGGg-GGCCCc -3' miRNA: 3'- caUACAUUGgua---CCGGUCCaaCCGGG- -5' |
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6103 | 3' | -52.6 | NC_001826.1 | + | 2566 | 1.13 | 0.002202 |
Target: 5'- aGUAUGUAACCAUGGCCAGGUUGGCCCa -3' miRNA: 3'- -CAUACAUUGGUACCGGUCCAACCGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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