Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6104 | 3' | -58.2 | NC_001826.1 | + | 112269 | 0.66 | 0.773729 |
Target: 5'- -cGCCACGCCC-CUcuaGaGAAGaUGGCg -3' miRNA: 3'- gaCGGUGCGGGuGAca-C-CUUCcACCG- -5' |
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6104 | 3' | -58.2 | NC_001826.1 | + | 2429 | 0.66 | 0.773729 |
Target: 5'- aUGCCAuauuuaUGCCUGUUGUaguuuaccaGGAAGGUGGUc -3' miRNA: 3'- gACGGU------GCGGGUGACA---------CCUUCCACCG- -5' |
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6104 | 3' | -58.2 | NC_001826.1 | + | 42077 | 0.66 | 0.773729 |
Target: 5'- -gGCCGCGCCCuucuugaaACU-UGGguGG-GGCa -3' miRNA: 3'- gaCGGUGCGGG--------UGAcACCuuCCaCCG- -5' |
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6104 | 3' | -58.2 | NC_001826.1 | + | 29636 | 0.66 | 0.753719 |
Target: 5'- -gGCCuuuGCGCCCucggcaaACgccGGggGGUGGUg -3' miRNA: 3'- gaCGG---UGCGGG-------UGacaCCuuCCACCG- -5' |
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6104 | 3' | -58.2 | NC_001826.1 | + | 113057 | 0.66 | 0.7352 |
Target: 5'- --uCCugGUCCuguaAUUGUGGuguGGUGGCa -3' miRNA: 3'- gacGGugCGGG----UGACACCuu-CCACCG- -5' |
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6104 | 3' | -58.2 | NC_001826.1 | + | 651 | 0.67 | 0.725319 |
Target: 5'- uCUGCuCACuGCCCGggccCUccGGGAGGUGaGCa -3' miRNA: 3'- -GACG-GUG-CGGGU----GAcaCCUUCCAC-CG- -5' |
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6104 | 3' | -58.2 | NC_001826.1 | + | 17071 | 0.67 | 0.715356 |
Target: 5'- gCUGUCAaaguCGCCCACUucccacucGUGGGccuGaGUGGCc -3' miRNA: 3'- -GACGGU----GCGGGUGA--------CACCUu--C-CACCG- -5' |
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6104 | 3' | -58.2 | NC_001826.1 | + | 3500 | 0.67 | 0.705322 |
Target: 5'- gUGCCACcuUCCACaGUGGGc-GUGGCa -3' miRNA: 3'- gACGGUGc-GGGUGaCACCUucCACCG- -5' |
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6104 | 3' | -58.2 | NC_001826.1 | + | 28935 | 0.67 | 0.705322 |
Target: 5'- -gGCCACuGCCCGCgUGUGGGuAGcucaGGCc -3' miRNA: 3'- gaCGGUG-CGGGUG-ACACCU-UCca--CCG- -5' |
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6104 | 3' | -58.2 | NC_001826.1 | + | 119047 | 0.67 | 0.684058 |
Target: 5'- -cGCCGCGCCCccCUGccgggggcUGGAcccccuccgccggGGGUGGg -3' miRNA: 3'- gaCGGUGCGGGu-GAC--------ACCU-------------UCCACCg -5' |
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6104 | 3' | -58.2 | NC_001826.1 | + | 25046 | 0.68 | 0.664651 |
Target: 5'- -aGCCAgCGCCUcuuACgugaugGUGGAAGGgcccgGGUg -3' miRNA: 3'- gaCGGU-GCGGG---UGa-----CACCUUCCa----CCG- -5' |
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6104 | 3' | -58.2 | NC_001826.1 | + | 38525 | 0.68 | 0.664651 |
Target: 5'- -aGCaGCGCuCCGCcGUGGAucuuggagacGGUGGCg -3' miRNA: 3'- gaCGgUGCG-GGUGaCACCUu---------CCACCG- -5' |
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6104 | 3' | -58.2 | NC_001826.1 | + | 24755 | 0.68 | 0.644121 |
Target: 5'- uUGCCACGUCUgaaGCggGUGGAgaGGGUcucacgGGCu -3' miRNA: 3'- gACGGUGCGGG---UGa-CACCU--UCCA------CCG- -5' |
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6104 | 3' | -58.2 | NC_001826.1 | + | 16911 | 0.69 | 0.552157 |
Target: 5'- -gGCCACucagGCCCACgaGUGGGAaGUGGg -3' miRNA: 3'- gaCGGUG----CGGGUGa-CACCUUcCACCg -5' |
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6104 | 3' | -58.2 | NC_001826.1 | + | 16024 | 0.72 | 0.401645 |
Target: 5'- cCU-CCACuGUCCAauCUGUGG-AGGUGGCg -3' miRNA: 3'- -GAcGGUG-CGGGU--GACACCuUCCACCG- -5' |
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6104 | 3' | -58.2 | NC_001826.1 | + | 118326 | 0.74 | 0.300271 |
Target: 5'- cCUGCC-UGCCCAgggGUGGggGGUaggGGCu -3' miRNA: 3'- -GACGGuGCGGGUga-CACCuuCCA---CCG- -5' |
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6104 | 3' | -58.2 | NC_001826.1 | + | 3647 | 1.12 | 0.000827 |
Target: 5'- gCUGCCACGCCCACUGUGGAAGGUGGCa -3' miRNA: 3'- -GACGGUGCGGGUGACACCUUCCACCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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