Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6105 | 3' | -44.4 | NC_001826.1 | + | 102523 | 0.66 | 0.99999 |
Target: 5'- -cAAGGAAGAGCUaCACcaguguuGUGAuuUAGUa -3' miRNA: 3'- caUUUCUUCUCGA-GUG-------UACUcuAUCG- -5' |
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6105 | 3' | -44.4 | NC_001826.1 | + | 106056 | 0.66 | 0.999986 |
Target: 5'- -aAGGGAAGaAGCUCugG-GAGGgaucaauUAGCa -3' miRNA: 3'- caUUUCUUC-UCGAGugUaCUCU-------AUCG- -5' |
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6105 | 3' | -44.4 | NC_001826.1 | + | 16672 | 0.66 | 0.999981 |
Target: 5'- -aGAGGAuuuGAGUacuuUCGCGugugUGGGGUGGCu -3' miRNA: 3'- caUUUCUu--CUCG----AGUGU----ACUCUAUCG- -5' |
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6105 | 3' | -44.4 | NC_001826.1 | + | 80003 | 0.66 | 0.999974 |
Target: 5'- aGUGAGGggGAuGCUUA-AUGGGAUcuguuuGGCc -3' miRNA: 3'- -CAUUUCuuCU-CGAGUgUACUCUA------UCG- -5' |
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6105 | 3' | -44.4 | NC_001826.1 | + | 118853 | 0.67 | 0.999964 |
Target: 5'- -gGAAGAGGGGCUCGggcccugcUcgGGGAU-GCc -3' miRNA: 3'- caUUUCUUCUCGAGU--------GuaCUCUAuCG- -5' |
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6105 | 3' | -44.4 | NC_001826.1 | + | 20168 | 0.67 | 0.999964 |
Target: 5'- -aGGAGGAGAGCUCugGaUGAuGAcAGa -3' miRNA: 3'- caUUUCUUCUCGAGugU-ACU-CUaUCg -5' |
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6105 | 3' | -44.4 | NC_001826.1 | + | 4480 | 0.67 | 0.999934 |
Target: 5'- --uAAGAuccAGGGCUCGC-UGGGAcGGUa -3' miRNA: 3'- cauUUCU---UCUCGAGUGuACUCUaUCG- -5' |
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6105 | 3' | -44.4 | NC_001826.1 | + | 41627 | 0.67 | 0.999934 |
Target: 5'- cUGGAGAGGAGUUCcaGCAUGAucacauucuuGGUuauGGCa -3' miRNA: 3'- cAUUUCUUCUCGAG--UGUACU----------CUA---UCG- -5' |
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6105 | 3' | -44.4 | NC_001826.1 | + | 29541 | 0.68 | 0.999736 |
Target: 5'- cUGGGGuAGAGUUCAUcaagugugGUGAGAUGGa -3' miRNA: 3'- cAUUUCuUCUCGAGUG--------UACUCUAUCg -5' |
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6105 | 3' | -44.4 | NC_001826.1 | + | 78570 | 0.69 | 0.999135 |
Target: 5'- aGUAAAGAucgaGGGGCUUcaaagucagaagACGcgGAGGUAGCc -3' miRNA: 3'- -CAUUUCU----UCUCGAG------------UGUa-CUCUAUCG- -5' |
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6105 | 3' | -44.4 | NC_001826.1 | + | 9249 | 0.7 | 0.998651 |
Target: 5'- aGUGccAGGAGGGGCUggUugAUGAGAuuuauggUAGCa -3' miRNA: 3'- -CAU--UUCUUCUCGA--GugUACUCU-------AUCG- -5' |
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6105 | 3' | -44.4 | NC_001826.1 | + | 114267 | 0.7 | 0.99803 |
Target: 5'- cUAAAGAAGAugcuGUUCGaggGGGGUAGCg -3' miRNA: 3'- cAUUUCUUCU----CGAGUguaCUCUAUCG- -5' |
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6105 | 3' | -44.4 | NC_001826.1 | + | 33168 | 0.7 | 0.99803 |
Target: 5'- -aAAAGAGGGGgUgGCAU-AGGUGGCa -3' miRNA: 3'- caUUUCUUCUCgAgUGUAcUCUAUCG- -5' |
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6105 | 3' | -44.4 | NC_001826.1 | + | 20624 | 0.71 | 0.995921 |
Target: 5'- -cAAGGAGGGGCcCACA-GGGcgGGCu -3' miRNA: 3'- caUUUCUUCUCGaGUGUaCUCuaUCG- -5' |
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6105 | 3' | -44.4 | NC_001826.1 | + | 52445 | 0.72 | 0.993345 |
Target: 5'- -gGAAGAAGAauGCUguCGUG-GGUGGCa -3' miRNA: 3'- caUUUCUUCU--CGAguGUACuCUAUCG- -5' |
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6105 | 3' | -44.4 | NC_001826.1 | + | 23163 | 0.74 | 0.974637 |
Target: 5'- uGUGGGGAAGA-CUCACggGGGGUGGa -3' miRNA: 3'- -CAUUUCUUCUcGAGUGuaCUCUAUCg -5' |
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6105 | 3' | -44.4 | NC_001826.1 | + | 3926 | 1.14 | 0.014961 |
Target: 5'- gGUAAAGAAGAGCUCACAUGAGAUAGCu -3' miRNA: 3'- -CAUUUCUUCUCGAGUGUACUCUAUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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