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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6106 | 3' | -55.1 | NC_001826.1 | + | 115032 | 0.67 | 0.868658 |
Target: 5'- uCUGGCcCACGCCaccauguacuGGAGGUC-UGGUUUc -3' miRNA: 3'- -GAUCGuGUGCGG----------CCUUCAGuGCCAGA- -5' |
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6106 | 3' | -55.1 | NC_001826.1 | + | 88030 | 0.68 | 0.810576 |
Target: 5'- -aGGCACGCGCauacuuuggccUGGAcauggucugcgaGGUUugGGUCUg -3' miRNA: 3'- gaUCGUGUGCG-----------GCCU------------UCAGugCCAGA- -5' |
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6106 | 3' | -55.1 | NC_001826.1 | + | 117525 | 0.68 | 0.791409 |
Target: 5'- -gGGCGCAUGCCcaggugacgucuuGaGAGGUCACGGg-- -3' miRNA: 3'- gaUCGUGUGCGG-------------C-CUUCAGUGCCaga -5' |
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6106 | 3' | -55.1 | NC_001826.1 | + | 29622 | 0.69 | 0.773497 |
Target: 5'- -cGGCAaACGCCGGGgggugguggcAGUCuAUGGUCa -3' miRNA: 3'- gaUCGUgUGCGGCCU----------UCAG-UGCCAGa -5' |
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6106 | 3' | -55.1 | NC_001826.1 | + | 5413 | 1.07 | 0.003585 |
Target: 5'- gCUAGCACACGCCGGAAGUCACGGUCUu -3' miRNA: 3'- -GAUCGUGUGCGGCCUUCAGUGCCAGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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