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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6107 | 3' | -59.4 | NC_001826.1 | + | 119053 | 0.68 | 0.546872 |
Target: 5'- -gGGGCCCGCcgcgccccCCUGCCGggggcUGGACCc -3' miRNA: 3'- caCUCGGGUGac------GGACGGU-----ACCUGGu -5' |
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6107 | 3' | -59.4 | NC_001826.1 | + | 28697 | 0.68 | 0.526828 |
Target: 5'- -aGAGgCaauGCUGCCaGCCAUGGACa- -3' miRNA: 3'- caCUCgGg--UGACGGaCGGUACCUGgu -5' |
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6107 | 3' | -59.4 | NC_001826.1 | + | 68030 | 0.7 | 0.448925 |
Target: 5'- gGUGAGauauguaCCCACauggaUGCCUGUUAUGGACUu -3' miRNA: 3'- -CACUC-------GGGUG-----ACGGACGGUACCUGGu -5' |
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6107 | 3' | -59.4 | NC_001826.1 | + | 87535 | 0.71 | 0.379477 |
Target: 5'- ----cCCCAacCUGgCUGCCGUGGACCAg -3' miRNA: 3'- cacucGGGU--GACgGACGGUACCUGGU- -5' |
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6107 | 3' | -59.4 | NC_001826.1 | + | 6512 | 1.08 | 0.00101 |
Target: 5'- cGUGAGCCCACUGCCUGCCAUGGACCAc -3' miRNA: 3'- -CACUCGGGUGACGGACGGUACCUGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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