Results 1 - 20 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6108 | 3' | -48.4 | NC_001826.1 | + | 103946 | 0.66 | 0.998439 |
Target: 5'- --gGAUGGCUGCuCGGGGucuguAGGggGCu -3' miRNA: 3'- cgaCUGCUGGCG-GUCCUuu---UUCuuUG- -5' |
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6108 | 3' | -48.4 | NC_001826.1 | + | 119448 | 0.66 | 0.99773 |
Target: 5'- gGCUGAgaGCCagGCgAGGGGAGAGggGg -3' miRNA: 3'- -CGACUgcUGG--CGgUCCUUUUUCuuUg -5' |
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6108 | 3' | -48.4 | NC_001826.1 | + | 118871 | 0.66 | 0.99773 |
Target: 5'- gGCU--CGACCcCCcaggGGGGAAGAGggGCu -3' miRNA: 3'- -CGAcuGCUGGcGG----UCCUUUUUCuuUG- -5' |
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6108 | 3' | -48.4 | NC_001826.1 | + | 108207 | 0.66 | 0.99773 |
Target: 5'- --aGGCGGCCGCgAGGGAcu-GuuGCu -3' miRNA: 3'- cgaCUGCUGGCGgUCCUUuuuCuuUG- -5' |
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6108 | 3' | -48.4 | NC_001826.1 | + | 32224 | 0.66 | 0.99773 |
Target: 5'- uGCUGAgGGCCGagcuggauaaCCuGGAaauccuagAAAGGggGCa -3' miRNA: 3'- -CGACUgCUGGC----------GGuCCU--------UUUUCuuUG- -5' |
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6108 | 3' | -48.4 | NC_001826.1 | + | 118622 | 0.66 | 0.99773 |
Target: 5'- uGCUGAgGGCCaaaaCAGGu-AGGGGAGCc -3' miRNA: 3'- -CGACUgCUGGcg--GUCCuuUUUCUUUG- -5' |
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6108 | 3' | -48.4 | NC_001826.1 | + | 118236 | 0.66 | 0.996769 |
Target: 5'- gGCUGAgaGCCagGCgaGGGGAGAGGggGCu -3' miRNA: 3'- -CGACUgcUGG--CGg-UCCUUUUUCuuUG- -5' |
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6108 | 3' | -48.4 | NC_001826.1 | + | 889 | 0.66 | 0.996769 |
Target: 5'- cCUGACaGCUGUCAGGGu-GAGAuuGGCa -3' miRNA: 3'- cGACUGcUGGCGGUCCUuuUUCU--UUG- -5' |
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6108 | 3' | -48.4 | NC_001826.1 | + | 82828 | 0.66 | 0.996542 |
Target: 5'- aGCUGAUGGCCGgCCAcagccauGAAGCu -3' miRNA: 3'- -CGACUGCUGGC-GGUccuuuuuCUUUG- -5' |
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6108 | 3' | -48.4 | NC_001826.1 | + | 103130 | 0.67 | 0.996176 |
Target: 5'- uGUUGugGGgUGCCGGGGAGcuuGGGuuCg -3' miRNA: 3'- -CGACugCUgGCGGUCCUUUu--UCUuuG- -5' |
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6108 | 3' | -48.4 | NC_001826.1 | + | 23936 | 0.67 | 0.99472 |
Target: 5'- cUUGugGGCUGUgAGGAcaaccuGGAGggGCu -3' miRNA: 3'- cGACugCUGGCGgUCCUu-----UUUCuuUG- -5' |
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6108 | 3' | -48.4 | NC_001826.1 | + | 119027 | 0.67 | 0.99384 |
Target: 5'- gGCUGGacccccuCCGCCGGGGGugggggguAGggGCa -3' miRNA: 3'- -CGACUgcu----GGCGGUCCUUuu------UCuuUG- -5' |
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6108 | 3' | -48.4 | NC_001826.1 | + | 28499 | 0.67 | 0.99384 |
Target: 5'- cGC-GGCGGCCGCCccggcGGGAcucgcagcuGGAAGggGu -3' miRNA: 3'- -CGaCUGCUGGCGG-----UCCU---------UUUUCuuUg -5' |
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6108 | 3' | -48.4 | NC_001826.1 | + | 756 | 0.67 | 0.992846 |
Target: 5'- cCUGACaGCUGUCAGGGGuuacauGAGAGAa -3' miRNA: 3'- cGACUGcUGGCGGUCCUUu-----UUCUUUg -5' |
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6108 | 3' | -48.4 | NC_001826.1 | + | 63799 | 0.68 | 0.991728 |
Target: 5'- --aGGCGAgUG-CAGGAAGAAGAAAg -3' miRNA: 3'- cgaCUGCUgGCgGUCCUUUUUCUUUg -5' |
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6108 | 3' | -48.4 | NC_001826.1 | + | 73241 | 0.68 | 0.991728 |
Target: 5'- uGUUGGCGGCCGaCCugauuAGGAGcaccAAGGAuaAGCa -3' miRNA: 3'- -CGACUGCUGGC-GG-----UCCUU----UUUCU--UUG- -5' |
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6108 | 3' | -48.4 | NC_001826.1 | + | 34284 | 0.7 | 0.971495 |
Target: 5'- aGCUG-CGACCcgaGUCaAGGAGAGGGGGAg -3' miRNA: 3'- -CGACuGCUGG---CGG-UCCUUUUUCUUUg -5' |
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6108 | 3' | -48.4 | NC_001826.1 | + | 28308 | 0.7 | 0.968351 |
Target: 5'- aGCUcgGGCGAggGCCGGGAAGgugGGGGAACc -3' miRNA: 3'- -CGA--CUGCUggCGGUCCUUU---UUCUUUG- -5' |
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6108 | 3' | -48.4 | NC_001826.1 | + | 87679 | 0.7 | 0.964969 |
Target: 5'- aGCUGguccACGGCaGCCAGGuuGGGGAGGg -3' miRNA: 3'- -CGAC----UGCUGgCGGUCCuuUUUCUUUg -5' |
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6108 | 3' | -48.4 | NC_001826.1 | + | 80660 | 0.72 | 0.934104 |
Target: 5'- uGCUGAUGgggaaGgUGCUGGGggGGAGGAGCc -3' miRNA: 3'- -CGACUGC-----UgGCGGUCCuuUUUCUUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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