miRNA: 3'- uucAC-UCGuCGGGGAACagUUUGGUC- -5'
miRNA: 3'- -UUCACUCgUCGGGGAaCAGuuUGGUC- -5'
miRNA: 3'- uUCAC-UCGUCGGGGAaCAGu-UUGGUC- -5'
miRNA: 3'- -UUCACUCGUCGGGGAACAGUUUGGUC- -5'
Results 1 - 4 of 4 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6109 | 5' | -53.4 | NC_001826.1 | + | 50958 | 0.66 | 0.928899 |
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Target: 5'- --uUGuAGU-GCCCCUUGagAAACCAGg -3' miRNA: 3'- uucAC-UCGuCGGGGAACagUUUGGUC- -5' |
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| 6109 | 5' | -53.4 | NC_001826.1 | + | 15399 | 0.67 | 0.876209 |
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Target: 5'- cAGGUGGGgAGCCUCU-GUC--ACCGGu -3' miRNA: 3'- -UUCACUCgUCGGGGAaCAGuuUGGUC- -5' |
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| 6109 | 5' | -53.4 | NC_001826.1 | + | 25337 | 0.69 | 0.808136 |
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Target: 5'- uAGUGuAGUAGCUCCUgGUUuuGGCCAGg -3' miRNA: 3'- uUCAC-UCGUCGGGGAaCAGu-UUGGUC- -5' |
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| 6109 | 5' | -53.4 | NC_001826.1 | + | 13314 | 1.07 | 0.003955 |
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Target: 5'- cAAGUGAGCAGCCCCUUGUCAAACCAGa -3' miRNA: 3'- -UUCACUCGUCGGGGAACAGUUUGGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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