Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
611 | 5' | -62.9 | AC_000017.1 | + | 33289 | 0.66 | 0.269939 |
Target: 5'- aCUGCUGCCG-CCGCCGCuccGUcCUgCAGg -3' miRNA: 3'- -GGCGACGGCgGGCGGUGc--CAcGA-GUC- -5' |
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611 | 5' | -62.9 | AC_000017.1 | + | 16217 | 0.66 | 0.269939 |
Target: 5'- cCCGgcaCUGCCGCCCaacgcgcgGCgGCGGcccUGCUUAa -3' miRNA: 3'- -GGC---GACGGCGGG--------CGgUGCC---ACGAGUc -5' |
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611 | 5' | -62.9 | AC_000017.1 | + | 17816 | 0.66 | 0.250372 |
Target: 5'- aCGCaUGCCGCCCGUCA-GGccgugGC-CGGc -3' miRNA: 3'- gGCG-ACGGCGGGCGGUgCCa----CGaGUC- -5' |
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611 | 5' | -62.9 | AC_000017.1 | + | 12143 | 0.66 | 0.248481 |
Target: 5'- gCCGCUGCCcguGCCaGCCAgGGcccuuugcaggcugUGCaUCAGc -3' miRNA: 3'- -GGCGACGG---CGGgCGGUgCC--------------ACG-AGUC- -5' |
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611 | 5' | -62.9 | AC_000017.1 | + | 8600 | 0.67 | 0.22612 |
Target: 5'- cCCGCggcccaCCGCCCGCCgcGCGGUaccguagucGCgccgCGGg -3' miRNA: 3'- -GGCGac----GGCGGGCGG--UGCCA---------CGa---GUC- -5' |
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611 | 5' | -62.9 | AC_000017.1 | + | 3668 | 0.68 | 0.188482 |
Target: 5'- gCGCaUGCC-CCCauggGCCGgGGUGCgUCAGa -3' miRNA: 3'- gGCG-ACGGcGGG----CGGUgCCACG-AGUC- -5' |
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611 | 5' | -62.9 | AC_000017.1 | + | 5366 | 0.69 | 0.152317 |
Target: 5'- -gGCUGCgCGCUgGCCAgGGUGCg--- -3' miRNA: 3'- ggCGACG-GCGGgCGGUgCCACGaguc -5' |
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611 | 5' | -62.9 | AC_000017.1 | + | 6706 | 0.71 | 0.112866 |
Target: 5'- gCGCuUGCCGCCCGCgCAUGGcagcagGCgUUAGa -3' miRNA: 3'- gGCG-ACGGCGGGCG-GUGCCa-----CG-AGUC- -5' |
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611 | 5' | -62.9 | AC_000017.1 | + | 15230 | 0.71 | 0.109796 |
Target: 5'- -aGCUGCCGCCCccGCUGCGGagGCUgCAc -3' miRNA: 3'- ggCGACGGCGGG--CGGUGCCa-CGA-GUc -5' |
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611 | 5' | -62.9 | AC_000017.1 | + | 26808 | 0.71 | 0.109796 |
Target: 5'- uUGCUGCCG-CUGCCGcCGGUGCagUAGg -3' miRNA: 3'- gGCGACGGCgGGCGGU-GCCACGa-GUC- -5' |
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611 | 5' | -62.9 | AC_000017.1 | + | 16330 | 0.72 | 0.092963 |
Target: 5'- cUCGCaugGCCGCCCGUCGgcCGGUGCg--- -3' miRNA: 3'- -GGCGa--CGGCGGGCGGU--GCCACGaguc -5' |
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611 | 5' | -62.9 | AC_000017.1 | + | 9799 | 1.09 | 0.000116 |
Target: 5'- cCCGCUGCCGCCCGCCACGGUGCUCAGc -3' miRNA: 3'- -GGCGACGGCGGGCGGUGCCACGAGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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