Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6110 | 5' | -56.3 | NC_001826.1 | + | 31121 | 0.66 | 0.826232 |
Target: 5'- -gCggGCugGGGGACGGGACC--CCu -3' miRNA: 3'- uaGaaCGugUCCUUGCCCUGGucGGu -5' |
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6110 | 5' | -56.3 | NC_001826.1 | + | 64834 | 0.66 | 0.808422 |
Target: 5'- uUCUgGgACAGGaucccuaucAugGGGGCCAGgCCAa -3' miRNA: 3'- uAGAaCgUGUCC---------UugCCCUGGUC-GGU- -5' |
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6110 | 5' | -56.3 | NC_001826.1 | + | 77478 | 0.66 | 0.799258 |
Target: 5'- ---aUGC-CAGGAugGGGaaGCCaauGGCCAg -3' miRNA: 3'- uagaACGuGUCCUugCCC--UGG---UCGGU- -5' |
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6110 | 5' | -56.3 | NC_001826.1 | + | 75970 | 0.69 | 0.669099 |
Target: 5'- ----aGUugAGGAgccaACGGGGCCAGuCCGg -3' miRNA: 3'- uagaaCGugUCCU----UGCCCUGGUC-GGU- -5' |
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6110 | 5' | -56.3 | NC_001826.1 | + | 102211 | 0.71 | 0.543363 |
Target: 5'- ----cGCGCAGGGGCGGGgacACUGGUCAa -3' miRNA: 3'- uagaaCGUGUCCUUGCCC---UGGUCGGU- -5' |
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6110 | 5' | -56.3 | NC_001826.1 | + | 13912 | 1.06 | 0.002598 |
Target: 5'- aAUCUUGCACAGGAACGGGACCAGCCAc -3' miRNA: 3'- -UAGAACGUGUCCUUGCCCUGGUCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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