Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6111 | 3' | -57.4 | NC_001826.1 | + | 118997 | 0.66 | 0.806548 |
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Target: 5'- gGGGcGCCAUGCGcccgcGCCUGgggCGCCAc -3' miRNA: 3'- aCCC-UGGUACGCcu---UGGACga-GUGGU- -5' |
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| 6111 | 3' | -57.4 | NC_001826.1 | + | 13897 | 0.66 | 0.806548 |
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Target: 5'- cGGGACCAgccacaUGCGGAuaguCCagGCUgGgCAu -3' miRNA: 3'- aCCCUGGU------ACGCCUu---GGa-CGAgUgGU- -5' |
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| 6111 | 3' | -57.4 | NC_001826.1 | + | 115105 | 0.66 | 0.797494 |
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Target: 5'- gGGGACCuGUGCGGGGauuucaaucUCUGC--ACCAc -3' miRNA: 3'- aCCCUGG-UACGCCUU---------GGACGagUGGU- -5' |
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| 6111 | 3' | -57.4 | NC_001826.1 | + | 119069 | 0.66 | 0.788287 |
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Target: 5'- cGGGGCgG-GgGGAACCgggGCcCGCCGc -3' miRNA: 3'- aCCCUGgUaCgCCUUGGa--CGaGUGGU- -5' |
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| 6111 | 3' | -57.4 | NC_001826.1 | + | 17315 | 0.66 | 0.759834 |
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Target: 5'- aGGGAuCCA--UGGAGCgUGC-CACCAu -3' miRNA: 3'- aCCCU-GGUacGCCUUGgACGaGUGGU- -5' |
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| 6111 | 3' | -57.4 | NC_001826.1 | + | 12497 | 0.67 | 0.750104 |
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Target: 5'- cUGGGACCcauucAUGCaGGAAUCUGgCaCGCCu -3' miRNA: 3'- -ACCCUGG-----UACG-CCUUGGAC-GaGUGGu -5' |
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| 6111 | 3' | -57.4 | NC_001826.1 | + | 8530 | 0.67 | 0.740267 |
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Target: 5'- aUGGGACCGUuaGGAAgacCCUGggCugCGu -3' miRNA: 3'- -ACCCUGGUAcgCCUU---GGACgaGugGU- -5' |
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| 6111 | 3' | -57.4 | NC_001826.1 | + | 41060 | 0.67 | 0.737297 |
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Target: 5'- aUGGGACCAUGCaaaaacuuuuGAACCUGguuggucaggcaacCUCGuCCAu -3' miRNA: 3'- -ACCCUGGUACGc---------CUUGGAC--------------GAGU-GGU- -5' |
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| 6111 | 3' | -57.4 | NC_001826.1 | + | 24207 | 0.68 | 0.638127 |
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Target: 5'- gGGGACC--GUGGAGgCUGCagCGCCu -3' miRNA: 3'- aCCCUGGuaCGCCUUgGACGa-GUGGu -5' |
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| 6111 | 3' | -57.4 | NC_001826.1 | + | 25812 | 1.08 | 0.001625 |
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Target: 5'- uUGGGACCAUGCGGAACCUGCUCACCAu -3' miRNA: 3'- -ACCCUGGUACGCCUUGGACGAGUGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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