Results 1 - 20 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6113 | 3' | -65.3 | NC_001826.1 | + | 21695 | 0.66 | 0.445789 |
Target: 5'- gUCUCCCugauuuugaaugacAACGaUgUCAGGGCGGCCGc -3' miRNA: 3'- aGGAGGG--------------UUGCcGgGGUCCCGCCGGU- -5' |
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6113 | 3' | -65.3 | NC_001826.1 | + | 28085 | 0.66 | 0.439698 |
Target: 5'- aCCUCCC--CGGCCCCcucccgagcuGGGGCccGGUUc -3' miRNA: 3'- aGGAGGGuuGCCGGGG----------UCCCG--CCGGu -5' |
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6113 | 3' | -65.3 | NC_001826.1 | + | 28126 | 0.66 | 0.439698 |
Target: 5'- aCCUCCC--CGGCCCCcucccgagcuGGGGCccGGUUc -3' miRNA: 3'- aGGAGGGuuGCCGGGG----------UCCCG--CCGGu -5' |
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6113 | 3' | -65.3 | NC_001826.1 | + | 20618 | 0.66 | 0.431079 |
Target: 5'- aCUUUUCAaggaGgGGCCCaCAGGGCgGGCUAa -3' miRNA: 3'- aGGAGGGU----UgCCGGG-GUCCCG-CCGGU- -5' |
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6113 | 3' | -65.3 | NC_001826.1 | + | 118451 | 0.66 | 0.414144 |
Target: 5'- cUCC-CCCGACcccaGCCCCcucGGCGGCUc -3' miRNA: 3'- -AGGaGGGUUGc---CGGGGuc-CCGCCGGu -5' |
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6113 | 3' | -65.3 | NC_001826.1 | + | 118765 | 0.66 | 0.397624 |
Target: 5'- aCC-CCCGGCgaacccGGCCCCGGGGacgcgggggaGGCUu -3' miRNA: 3'- aGGaGGGUUG------CCGGGGUCCCg---------CCGGu -5' |
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6113 | 3' | -65.3 | NC_001826.1 | + | 118947 | 0.67 | 0.381533 |
Target: 5'- cCCggCCCGccGCGGCCCguccuCAGGcccgggcgguaGCGGCCGc -3' miRNA: 3'- aGGa-GGGU--UGCCGGG-----GUCC-----------CGCCGGU- -5' |
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6113 | 3' | -65.3 | NC_001826.1 | + | 4624 | 0.67 | 0.381533 |
Target: 5'- aCCgUCCCAGCGaGCCCUGGaucuuaGGCCAa -3' miRNA: 3'- aGG-AGGGUUGC-CGGGGUCccg---CCGGU- -5' |
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6113 | 3' | -65.3 | NC_001826.1 | + | 31585 | 0.67 | 0.381533 |
Target: 5'- uUCUUCUCuguuACcugCCCAGGGCGGCCu -3' miRNA: 3'- -AGGAGGGu---UGccgGGGUCCCGCCGGu -5' |
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6113 | 3' | -65.3 | NC_001826.1 | + | 114876 | 0.67 | 0.365882 |
Target: 5'- aCUUUCC-AUGGCCCCAGGGa-GCUc -3' miRNA: 3'- aGGAGGGuUGCCGGGGUCCCgcCGGu -5' |
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6113 | 3' | -65.3 | NC_001826.1 | + | 34008 | 0.67 | 0.365882 |
Target: 5'- -aCUCagCAGCaGCCuCCAGGGCGGUg- -3' miRNA: 3'- agGAGg-GUUGcCGG-GGUCCCGCCGgu -5' |
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6113 | 3' | -65.3 | NC_001826.1 | + | 118948 | 0.68 | 0.321642 |
Target: 5'- cCCUCCC--CGGgCCCGGGGCccCCAc -3' miRNA: 3'- aGGAGGGuuGCCgGGGUCCCGccGGU- -5' |
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6113 | 3' | -65.3 | NC_001826.1 | + | 2552 | 0.68 | 0.301068 |
Target: 5'- gCCagguuggCCCAGUGGCguuUCCAGGGCcGGCCAg -3' miRNA: 3'- aGGa------GGGUUGCCG---GGGUCCCG-CCGGU- -5' |
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6113 | 3' | -65.3 | NC_001826.1 | + | 118921 | 0.69 | 0.281525 |
Target: 5'- gCCUCCCccGCGuCCCCGGGGCcggguucGCCGg -3' miRNA: 3'- aGGAGGGu-UGCcGGGGUCCCGc------CGGU- -5' |
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6113 | 3' | -65.3 | NC_001826.1 | + | 119394 | 0.69 | 0.273373 |
Target: 5'- cUCCUCCUGGCGcccccuacuguuguGUCCaggggCAGGGUGGCCGc -3' miRNA: 3'- -AGGAGGGUUGC--------------CGGG-----GUCCCGCCGGU- -5' |
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6113 | 3' | -65.3 | NC_001826.1 | + | 119133 | 0.7 | 0.24548 |
Target: 5'- -gCgggCgCAugGcGCCCCAGGcGCGGCCGc -3' miRNA: 3'- agGa--GgGUugC-CGGGGUCC-CGCCGGU- -5' |
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6113 | 3' | -65.3 | NC_001826.1 | + | 37471 | 0.7 | 0.228939 |
Target: 5'- aCCUCCCAGUGGCuCCCgAGGuGCuaGGCCc -3' miRNA: 3'- aGGAGGGUUGCCG-GGG-UCC-CG--CCGGu -5' |
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6113 | 3' | -65.3 | NC_001826.1 | + | 69480 | 0.71 | 0.21335 |
Target: 5'- ---gCCCGggGCGGCCCUuGGuGCGGCCGa -3' miRNA: 3'- aggaGGGU--UGCCGGGGuCC-CGCCGGU- -5' |
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6113 | 3' | -65.3 | NC_001826.1 | + | 101988 | 0.71 | 0.203471 |
Target: 5'- cCCUCCCcACcgaaCCCCGGGGCGGUUu -3' miRNA: 3'- aGGAGGGuUGcc--GGGGUCCCGCCGGu -5' |
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6113 | 3' | -65.3 | NC_001826.1 | + | 119179 | 0.72 | 0.184899 |
Target: 5'- gCC-CCCAGCcccgGGUCuCCAGGGCGcGCCGc -3' miRNA: 3'- aGGaGGGUUG----CCGG-GGUCCCGC-CGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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