Results 21 - 23 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6113 | 3' | -65.3 | NC_001826.1 | + | 31585 | 0.67 | 0.381533 |
Target: 5'- uUCUUCUCuguuACcugCCCAGGGCGGCCu -3' miRNA: 3'- -AGGAGGGu---UGccgGGGUCCCGCCGGu -5' |
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6113 | 3' | -65.3 | NC_001826.1 | + | 4624 | 0.67 | 0.381533 |
Target: 5'- aCCgUCCCAGCGaGCCCUGGaucuuaGGCCAa -3' miRNA: 3'- aGG-AGGGUUGC-CGGGGUCccg---CCGGU- -5' |
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6113 | 3' | -65.3 | NC_001826.1 | + | 29196 | 1.08 | 0.000374 |
Target: 5'- gUCCUCCCAACGGCCCCAGGGCGGCCAg -3' miRNA: 3'- -AGGAGGGUUGCCGGGGUCCCGCCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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