Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6114 | 3' | -48 | NC_001826.1 | + | 94641 | 0.66 | 0.998837 |
Target: 5'- cCCAccaUGUCAUAcacuguaaacucUGUCGCCCCA-CAa -3' miRNA: 3'- -GGUug-AUAGUGU------------AUAGCGGGGUuGUa -5' |
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6114 | 3' | -48 | NC_001826.1 | + | 119140 | 0.66 | 0.998579 |
Target: 5'- cCCAggcGCgggCGCAUggCGCCCCAggcGCGg -3' miRNA: 3'- -GGU---UGauaGUGUAuaGCGGGGU---UGUa -5' |
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6114 | 3' | -48 | NC_001826.1 | + | 4014 | 0.68 | 0.993268 |
Target: 5'- aCCuucACUGUUACuccUCGCCCCAcuccACAa -3' miRNA: 3'- -GGu--UGAUAGUGuauAGCGGGGU----UGUa -5' |
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6114 | 3' | -48 | NC_001826.1 | + | 57626 | 0.68 | 0.989634 |
Target: 5'- cUCGGgaAUCAUAUAcauagaCGCCCCGACAg -3' miRNA: 3'- -GGUUgaUAGUGUAUa-----GCGGGGUUGUa -5' |
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6114 | 3' | -48 | NC_001826.1 | + | 85420 | 0.7 | 0.975311 |
Target: 5'- --cGCUAUCACcgAcaCGCCCCAGCu- -3' miRNA: 3'- gguUGAUAGUGuaUa-GCGGGGUUGua -5' |
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6114 | 3' | -48 | NC_001826.1 | + | 72887 | 0.71 | 0.950132 |
Target: 5'- cUCAuCUA-CACuUAUUGCCCCAGCAg -3' miRNA: 3'- -GGUuGAUaGUGuAUAGCGGGGUUGUa -5' |
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6114 | 3' | -48 | NC_001826.1 | + | 30238 | 0.72 | 0.918168 |
Target: 5'- gCCAGCc-UCugGUAUCGCCUgAACAc -3' miRNA: 3'- -GGUUGauAGugUAUAGCGGGgUUGUa -5' |
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6114 | 3' | -48 | NC_001826.1 | + | 30071 | 1.09 | 0.012466 |
Target: 5'- uCCAACUAUCACAUAUCGCCCCAACAUg -3' miRNA: 3'- -GGUUGAUAGUGUAUAGCGGGGUUGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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