Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6116 | 5' | -56.3 | NC_001826.1 | + | 54390 | 0.66 | 0.82119 |
Target: 5'- cGGGACgUGACCCCagauauaaaguaUGCAGuGU-GCCa -3' miRNA: 3'- aCCUUG-ACUGGGG------------ACGUCuCAgUGGc -5' |
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6116 | 5' | -56.3 | NC_001826.1 | + | 69731 | 0.66 | 0.82119 |
Target: 5'- cGGAgGCUGACCCCaaaGCGG---CACCGu -3' miRNA: 3'- aCCU-UGACUGGGGa--CGUCucaGUGGC- -5' |
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6116 | 5' | -56.3 | NC_001826.1 | + | 118256 | 0.67 | 0.794318 |
Target: 5'- gGGGGCUGGaggggggaagaCCCUGCcagGGAGUC-CCa -3' miRNA: 3'- aCCUUGACUg----------GGGACG---UCUCAGuGGc -5' |
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6116 | 5' | -56.3 | NC_001826.1 | + | 28241 | 0.67 | 0.794318 |
Target: 5'- gGGAACcgGGCCCCaGCucgGGAGgggGCCGg -3' miRNA: 3'- aCCUUGa-CUGGGGaCG---UCUCag-UGGC- -5' |
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6116 | 5' | -56.3 | NC_001826.1 | + | 28282 | 0.67 | 0.794318 |
Target: 5'- gGGAACcgGGCCCCaGCucgGGAGgggGCCGg -3' miRNA: 3'- aCCUUGa-CUGGGGaCG---UCUCag-UGGC- -5' |
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6116 | 5' | -56.3 | NC_001826.1 | + | 5221 | 0.68 | 0.726767 |
Target: 5'- cGGGgcGCUGACUgCUGCAGAacugccaaGUCugCa -3' miRNA: 3'- aCCU--UGACUGGgGACGUCU--------CAGugGc -5' |
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6116 | 5' | -56.3 | NC_001826.1 | + | 31001 | 0.68 | 0.723756 |
Target: 5'- aGGAACUGAucagggccaaacagUCCCUG-AGAGUCAgCu -3' miRNA: 3'- aCCUUGACU--------------GGGGACgUCUCAGUgGc -5' |
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6116 | 5' | -56.3 | NC_001826.1 | + | 43490 | 0.69 | 0.706563 |
Target: 5'- aUGGGGuacagcucaacuCUGAUUCCUGCuAGAGUUGCUGg -3' miRNA: 3'- -ACCUU------------GACUGGGGACG-UCUCAGUGGC- -5' |
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6116 | 5' | -56.3 | NC_001826.1 | + | 91542 | 0.69 | 0.675792 |
Target: 5'- gUGGAAUUGAUa--UGCGGuGUCACCa -3' miRNA: 3'- -ACCUUGACUGgggACGUCuCAGUGGc -5' |
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6116 | 5' | -56.3 | NC_001826.1 | + | 118544 | 0.7 | 0.634289 |
Target: 5'- cGGAGCUGGCCCU--CGGAG--GCCGg -3' miRNA: 3'- aCCUUGACUGGGGacGUCUCagUGGC- -5' |
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6116 | 5' | -56.3 | NC_001826.1 | + | 22282 | 0.73 | 0.482212 |
Target: 5'- cUGGuGCgUGACCUg-GCAGGGUCACCa -3' miRNA: 3'- -ACCuUG-ACUGGGgaCGUCUCAGUGGc -5' |
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6116 | 5' | -56.3 | NC_001826.1 | + | 118302 | 0.74 | 0.408407 |
Target: 5'- aGGGGCUGGgcCCCCUgGCAGGGagGCUGg -3' miRNA: 3'- aCCUUGACU--GGGGA-CGUCUCagUGGC- -5' |
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6116 | 5' | -56.3 | NC_001826.1 | + | 32579 | 1.08 | 0.0023 |
Target: 5'- uUGGAACUGACCCCUGCAGAGUCACCGc -3' miRNA: 3'- -ACCUUGACUGGGGACGUCUCAGUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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