Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6117 | 3' | -50.2 | NC_001826.1 | + | 41626 | 0.66 | 0.988731 |
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Target: 5'- aGGAGGCGAu--CCUccCUCCAGc-- -3' miRNA: 3'- aCUUCCGCUuauGGGuaGAGGUCauc -5' |
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| 6117 | 3' | -50.2 | NC_001826.1 | + | 57208 | 0.66 | 0.988731 |
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Target: 5'- uUGAGGGCaguGAuUACCCAUauaCCAGUu- -3' miRNA: 3'- -ACUUCCG---CUuAUGGGUAga-GGUCAuc -5' |
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| 6117 | 3' | -50.2 | NC_001826.1 | + | 118838 | 0.66 | 0.98542 |
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Target: 5'- cGggGG-GAGUGCCCcUC-CCGGggagGGg -3' miRNA: 3'- aCuuCCgCUUAUGGGuAGaGGUCa---UC- -5' |
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| 6117 | 3' | -50.2 | NC_001826.1 | + | 115139 | 0.66 | 0.98542 |
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Target: 5'- ---cGGCGAccgcucAUugCCAUUUCCAGaAGa -3' miRNA: 3'- acuuCCGCU------UAugGGUAGAGGUCaUC- -5' |
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| 6117 | 3' | -50.2 | NC_001826.1 | + | 23801 | 0.67 | 0.979122 |
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Target: 5'- -aAGGGCGGaccccacgGUGCCCGcgUCUuCCAGUAu -3' miRNA: 3'- acUUCCGCU--------UAUGGGU--AGA-GGUCAUc -5' |
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| 6117 | 3' | -50.2 | NC_001826.1 | + | 21773 | 0.69 | 0.94843 |
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Target: 5'- aGGAGGgGccUGCCCAguggCUUCAGUAa -3' miRNA: 3'- aCUUCCgCuuAUGGGUa---GAGGUCAUc -5' |
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| 6117 | 3' | -50.2 | NC_001826.1 | + | 114127 | 0.69 | 0.938855 |
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Target: 5'- cGggGGCGGu--UCCAUCUgCAGUu- -3' miRNA: 3'- aCuuCCGCUuauGGGUAGAgGUCAuc -5' |
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| 6117 | 3' | -50.2 | NC_001826.1 | + | 62374 | 0.7 | 0.928209 |
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Target: 5'- -aGAGGCGAGUccaGCCaaCAUaCUCUAGUGGg -3' miRNA: 3'- acUUCCGCUUA---UGG--GUA-GAGGUCAUC- -5' |
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| 6117 | 3' | -50.2 | NC_001826.1 | + | 118133 | 0.74 | 0.736591 |
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Target: 5'- aGAGGGgGGGgggGCUCAUCUCCAGc-- -3' miRNA: 3'- aCUUCCgCUUa--UGGGUAGAGGUCauc -5' |
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| 6117 | 3' | -50.2 | NC_001826.1 | + | 50344 | 1.09 | 0.007509 |
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Target: 5'- aUGAAGGCGAAUACCCAUCUCCAGUAGc -3' miRNA: 3'- -ACUUCCGCUUAUGGGUAGAGGUCAUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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