Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6118 | 5' | -55.5 | NC_001826.1 | + | 65720 | 0.66 | 0.848873 |
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Target: 5'- aCGugCU---GCUGGCcacgUCCAGGUACuCg -3' miRNA: 3'- -GCugGGauuCGACCG----AGGUCCAUGuG- -5' |
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| 6118 | 5' | -55.5 | NC_001826.1 | + | 70287 | 0.67 | 0.840603 |
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Target: 5'- gGACUgUcAGC-GGUUUCAGGUACAUc -3' miRNA: 3'- gCUGGgAuUCGaCCGAGGUCCAUGUG- -5' |
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| 6118 | 5' | -55.5 | NC_001826.1 | + | 118612 | 0.67 | 0.823469 |
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Target: 5'- cCGACCCccggGGGCgGGC-CCGGGgcuUACu -3' miRNA: 3'- -GCUGGGa---UUCGaCCGaGGUCCau-GUG- -5' |
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| 6118 | 5' | -55.5 | NC_001826.1 | + | 10480 | 0.67 | 0.823469 |
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Target: 5'- uGGCCCUcaguauauAGGCUGGggacuugaCUUCgggAGGUGCACa -3' miRNA: 3'- gCUGGGA--------UUCGACC--------GAGG---UCCAUGUG- -5' |
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| 6118 | 5' | -55.5 | NC_001826.1 | + | 118762 | 0.68 | 0.767945 |
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Target: 5'- cCGACCCccGGCgaacccGGCcCCGGGgACGCg -3' miRNA: 3'- -GCUGGGauUCGa-----CCGaGGUCCaUGUG- -5' |
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| 6118 | 5' | -55.5 | NC_001826.1 | + | 30591 | 0.68 | 0.767945 |
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Target: 5'- aGugCCUGAcCcaaGGCUCCAGGccuUGCGCu -3' miRNA: 3'- gCugGGAUUcGa--CCGAGGUCC---AUGUG- -5' |
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| 6118 | 5' | -55.5 | NC_001826.1 | + | 32403 | 0.68 | 0.748312 |
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Target: 5'- aGACCCc--GCUGGCUCuuaaauauCAGGUGUGCg -3' miRNA: 3'- gCUGGGauuCGACCGAG--------GUCCAUGUG- -5' |
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| 6118 | 5' | -55.5 | NC_001826.1 | + | 114297 | 0.72 | 0.540949 |
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Target: 5'- -aGCCCUGGGCUGauuGCUgCCAGGUuuGCa -3' miRNA: 3'- gcUGGGAUUCGAC---CGA-GGUCCAugUG- -5' |
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| 6118 | 5' | -55.5 | NC_001826.1 | + | 118493 | 0.74 | 0.452225 |
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Target: 5'- uGGCCCUcAGCaGGgUCCGGGUGCc- -3' miRNA: 3'- gCUGGGAuUCGaCCgAGGUCCAUGug -5' |
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| 6118 | 5' | -55.5 | NC_001826.1 | + | 74384 | 1.11 | 0.001614 |
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Target: 5'- aCGACCCUAAGCUGGCUCCAGGUACACg -3' miRNA: 3'- -GCUGGGAUUCGACCGAGGUCCAUGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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