Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6119 | 3' | -53.5 | NC_001826.1 | + | 91828 | 0.66 | 0.944828 |
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Target: 5'- aCAGCCUGU-ACCAggaaucUGCCACCUUUa- -3' miRNA: 3'- cGUCGGGUAgUGGU------AUGGUGGAAGca -5' |
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| 6119 | 3' | -53.5 | NC_001826.1 | + | 90328 | 0.67 | 0.917966 |
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Target: 5'- -gGGCCgCAUCuACCAUGCUuguaaaaACCUUCa- -3' miRNA: 3'- cgUCGG-GUAG-UGGUAUGG-------UGGAAGca -5' |
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| 6119 | 3' | -53.5 | NC_001826.1 | + | 104689 | 0.67 | 0.906252 |
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Target: 5'- ---cCCCAUCACCAccagUGCCcCCUUCa- -3' miRNA: 3'- cgucGGGUAGUGGU----AUGGuGGAAGca -5' |
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| 6119 | 3' | -53.5 | NC_001826.1 | + | 48630 | 0.68 | 0.863393 |
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Target: 5'- cCA-CCCGUCACCAggcucaaaaaaACCGCCUUUGa -3' miRNA: 3'- cGUcGGGUAGUGGUa----------UGGUGGAAGCa -5' |
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| 6119 | 3' | -53.5 | NC_001826.1 | + | 3467 | 0.68 | 0.861028 |
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Target: 5'- uCAGUCCAuuaaguggaugcugUCACCuacugaGUGCCACCUUCc- -3' miRNA: 3'- cGUCGGGU--------------AGUGG------UAUGGUGGAAGca -5' |
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| 6119 | 3' | -53.5 | NC_001826.1 | + | 20397 | 0.69 | 0.830269 |
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Target: 5'- -aAGCUgGUCACCAUGaggACCUUCGa -3' miRNA: 3'- cgUCGGgUAGUGGUAUgg-UGGAAGCa -5' |
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| 6119 | 3' | -53.5 | NC_001826.1 | + | 17504 | 0.71 | 0.724998 |
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Target: 5'- uGUGGCCaaugaaGUCACC--GCCACCUUCa- -3' miRNA: 3'- -CGUCGGg-----UAGUGGuaUGGUGGAAGca -5' |
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| 6119 | 3' | -53.5 | NC_001826.1 | + | 63423 | 0.79 | 0.32647 |
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Target: 5'- uGCAGCCCAgCACCAUugaugaGCCAUCUUCc- -3' miRNA: 3'- -CGUCGGGUaGUGGUA------UGGUGGAAGca -5' |
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| 6119 | 3' | -53.5 | NC_001826.1 | + | 79082 | 1.1 | 0.003088 |
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Target: 5'- uGCAGCCCAUCACCAUACCACCUUCGUg -3' miRNA: 3'- -CGUCGGGUAGUGGUAUGGUGGAAGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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