Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 612 | 5' | -60.9 | AC_000017.1 | + | 12206 | 0.66 | 0.287808 |
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Target: 5'- gCGUCGGCUuggGGCCCaGCgcaggUCAGCg- -3' miRNA: 3'- aGCGGCUGGa--CCGGGaCGa----AGUCGgu -5' |
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| 612 | 5' | -60.9 | AC_000017.1 | + | 3163 | 0.67 | 0.266668 |
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Target: 5'- cUCGCaaGGCCUGGCCagUGUUUgAGCaCAa -3' miRNA: 3'- -AGCGg-CUGGACCGGg-ACGAAgUCG-GU- -5' |
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| 612 | 5' | -60.9 | AC_000017.1 | + | 28055 | 0.67 | 0.246802 |
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Target: 5'- aCGCCGugCgccgGGCCCucgauaUGCUcuUCGGgCAa -3' miRNA: 3'- aGCGGCugGa---CCGGG------ACGA--AGUCgGU- -5' |
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| 612 | 5' | -60.9 | AC_000017.1 | + | 2238 | 0.67 | 0.234251 |
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Target: 5'- cCGCCGccGCCUGGCuuccuCCUGCUgCuGCUg -3' miRNA: 3'- aGCGGC--UGGACCG-----GGACGAaGuCGGu -5' |
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| 612 | 5' | -60.9 | AC_000017.1 | + | 34169 | 0.68 | 0.22224 |
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Target: 5'- aCGcCCGcACCUGGUuuUGCUUCAGg-- -3' miRNA: 3'- aGC-GGC-UGGACCGggACGAAGUCggu -5' |
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| 612 | 5' | -60.9 | AC_000017.1 | + | 5419 | 0.74 | 0.074927 |
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Target: 5'- cUGCCGGuCUUcGCCCUGCgcgUCGGCCAg -3' miRNA: 3'- aGCGGCU-GGAcCGGGACGa--AGUCGGU- -5' |
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| 612 | 5' | -60.9 | AC_000017.1 | + | 12584 | 0.77 | 0.04702 |
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Target: 5'- -gGCCGGCCUGGUCuacgacgcgCUGCUUCAGCg- -3' miRNA: 3'- agCGGCUGGACCGG---------GACGAAGUCGgu -5' |
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| 612 | 5' | -60.9 | AC_000017.1 | + | 12626 | 0.79 | 0.033917 |
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Target: 5'- -gGCCGGCCucaucgggccggaUGGCCCUGuUUUCGGCCAg -3' miRNA: 3'- agCGGCUGG-------------ACCGGGAC-GAAGUCGGU- -5' |
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| 612 | 5' | -60.9 | AC_000017.1 | + | 10145 | 1.08 | 0.000151 |
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Target: 5'- gUCGCCGACCUGGCCCUGCUUCAGCCAa -3' miRNA: 3'- -AGCGGCUGGACCGGGACGAAGUCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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