Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6120 | 3' | -49.9 | NC_001826.1 | + | 111875 | 0.66 | 0.991377 |
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Target: 5'- cAUCUugggGUCCACCUAUggCUUAgUGGAAGc -3' miRNA: 3'- -UAGA----CAGGUGGAUGa-GAGU-ACCUUUc -5' |
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| 6120 | 3' | -49.9 | NC_001826.1 | + | 17390 | 0.67 | 0.983319 |
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Target: 5'- cUCUG-CCACUUACUC-C-UGGAAAa -3' miRNA: 3'- uAGACaGGUGGAUGAGaGuACCUUUc -5' |
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| 6120 | 3' | -49.9 | NC_001826.1 | + | 63979 | 0.69 | 0.951969 |
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Target: 5'- gAUgaGUCCACCaGCUCUUcUGGcGAGg -3' miRNA: 3'- -UAgaCAGGUGGaUGAGAGuACCuUUC- -5' |
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| 6120 | 3' | -49.9 | NC_001826.1 | + | 19092 | 0.7 | 0.914804 |
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Target: 5'- uUCUGUCCACCUuaucuuguuguGCUCUCuuuUGuuGAGu -3' miRNA: 3'- uAGACAGGUGGA-----------UGAGAGu--ACcuUUC- -5' |
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| 6120 | 3' | -49.9 | NC_001826.1 | + | 7295 | 0.74 | 0.762618 |
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Target: 5'- cUCUGUCUACCcACUCUUcUGGAGu- -3' miRNA: 3'- uAGACAGGUGGaUGAGAGuACCUUuc -5' |
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| 6120 | 3' | -49.9 | NC_001826.1 | + | 114081 | 0.8 | 0.43412 |
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Target: 5'- uGUCUGcaCCACCUGCUCUUAUGGGc-- -3' miRNA: 3'- -UAGACa-GGUGGAUGAGAGUACCUuuc -5' |
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| 6120 | 3' | -49.9 | NC_001826.1 | + | 82215 | 1.08 | 0.008382 |
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Target: 5'- gAUCUGUCCACCUACUCUCAUGGAAAGa -3' miRNA: 3'- -UAGACAGGUGGAUGAGAGUACCUUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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