Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6123 | 5' | -51.7 | NC_001826.1 | + | 11977 | 0.66 | 0.961921 |
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Target: 5'- aUGCGcACagcuUGGGCCAcguuGGUguaGUAGCUCu -3' miRNA: 3'- gACGUaUG----ACCCGGUu---UCG---UAUCGAG- -5' |
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| 6123 | 5' | -51.7 | NC_001826.1 | + | 118305 | 0.66 | 0.958083 |
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Target: 5'- -gGUAgggGCUGGGCCcccuGGCAgggaGGCUg -3' miRNA: 3'- gaCGUa--UGACCCGGuu--UCGUa---UCGAg -5' |
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| 6123 | 5' | -51.7 | NC_001826.1 | + | 85566 | 0.67 | 0.940119 |
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Target: 5'- cCUGCA-GCUGGGgCGugucggugauAGCGaAGCUCa -3' miRNA: 3'- -GACGUaUGACCCgGUu---------UCGUaUCGAG- -5' |
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| 6123 | 5' | -51.7 | NC_001826.1 | + | 77806 | 0.67 | 0.923805 |
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Target: 5'- uUGCAUAUUGGG--GGAGUGUAuGCUCu -3' miRNA: 3'- gACGUAUGACCCggUUUCGUAU-CGAG- -5' |
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| 6123 | 5' | -51.7 | NC_001826.1 | + | 16750 | 0.68 | 0.917818 |
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Target: 5'- -aGCGUcCUGGGCUggGGUcaugucugAUGGCUg -3' miRNA: 3'- gaCGUAuGACCCGGuuUCG--------UAUCGAg -5' |
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| 6123 | 5' | -51.7 | NC_001826.1 | + | 70688 | 0.68 | 0.905025 |
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Target: 5'- -gGgGUGCUGGGUUcuGGGGUAgagGGCUCa -3' miRNA: 3'- gaCgUAUGACCCGG--UUUCGUa--UCGAG- -5' |
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| 6123 | 5' | -51.7 | NC_001826.1 | + | 26410 | 0.71 | 0.787892 |
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Target: 5'- uUGCu--CUGGGCCAauGAGCA-GGCUa -3' miRNA: 3'- gACGuauGACCCGGU--UUCGUaUCGAg -5' |
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| 6123 | 5' | -51.7 | NC_001826.1 | + | 100 | 0.75 | 0.52824 |
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Target: 5'- uCUGCAaagcuugcuuuuugUGCUGGuGCCAGAGU--AGCUCa -3' miRNA: 3'- -GACGU--------------AUGACC-CGGUUUCGuaUCGAG- -5' |
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| 6123 | 5' | -51.7 | NC_001826.1 | + | 2049 | 1.11 | 0.003041 |
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Target: 5'- cCUGCAUACUGGGCCAAAGCAUAGCUCa -3' miRNA: 3'- -GACGUAUGACCCGGUUUCGUAUCGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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