Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6124 | 5' | -57.5 | NC_001826.1 | + | 119126 | 0.66 | 0.821528 |
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Target: 5'- cAUGGCG-CCCCAGgcgcggcCGCUaccgcccgggccugaGGACGGg -3' miRNA: 3'- aUGCCGCaGGGGUCa------GUGAg--------------UCUGCC- -5' |
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| 6124 | 5' | -57.5 | NC_001826.1 | + | 118957 | 0.67 | 0.775828 |
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Target: 5'- cGCGGCccGUCCUCAG--GCcCGGGCGGu -3' miRNA: 3'- aUGCCG--CAGGGGUCagUGaGUCUGCC- -5' |
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| 6124 | 5' | -57.5 | NC_001826.1 | + | 70760 | 0.68 | 0.704912 |
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Target: 5'- gGCGGUGUCgCCUcuggugucauagauGGUcCACUUGGugGGg -3' miRNA: 3'- aUGCCGCAG-GGG--------------UCA-GUGAGUCugCC- -5' |
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| 6124 | 5' | -57.5 | NC_001826.1 | + | 33308 | 0.68 | 0.697884 |
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Target: 5'- gGCGcGCGUCCUCGGUC-C-CGGGCa- -3' miRNA: 3'- aUGC-CGCAGGGGUCAGuGaGUCUGcc -5' |
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| 6124 | 5' | -57.5 | NC_001826.1 | + | 112851 | 0.68 | 0.696877 |
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Target: 5'- aUugGGCGaauaaaccCCCCAGUCACUggaacacCAGAUGc -3' miRNA: 3'- -AugCCGCa-------GGGGUCAGUGA-------GUCUGCc -5' |
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| 6124 | 5' | -57.5 | NC_001826.1 | + | 10875 | 1.08 | 0.002044 |
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Target: 5'- cUACGGCGUCCCCAGUCACUCAGACGGu -3' miRNA: 3'- -AUGCCGCAGGGGUCAGUGAGUCUGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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