Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6125 | 3' | -52.9 | NC_001826.1 | + | 16743 | 0.66 | 0.948408 |
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Target: 5'- uGGGCUGGGGUCAuguCUGAugGCugccuCAGa -3' miRNA: 3'- -CUCGGCCCCGGU---GACUuuUGuau--GUC- -5' |
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| 6125 | 3' | -52.9 | NC_001826.1 | + | 118553 | 0.66 | 0.94382 |
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Target: 5'- -cGCCGGGGCCGgaGcuGGCccuCGGa -3' miRNA: 3'- cuCGGCCCCGGUgaCuuUUGuauGUC- -5' |
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| 6125 | 3' | -52.9 | NC_001826.1 | + | 5110 | 0.67 | 0.903734 |
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Target: 5'- -uGCUGuGGGaCACUGAAAuugcugugcuacaGCGUGCAGa -3' miRNA: 3'- cuCGGC-CCCgGUGACUUU-------------UGUAUGUC- -5' |
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| 6125 | 3' | -52.9 | NC_001826.1 | + | 25649 | 0.68 | 0.890788 |
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Target: 5'- cAGCCGGGGCUA--GAGcACAUGguGa -3' miRNA: 3'- cUCGGCCCCGGUgaCUUuUGUAUguC- -5' |
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| 6125 | 3' | -52.9 | NC_001826.1 | + | 118587 | 0.68 | 0.88361 |
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Target: 5'- gGGGCUGGGGUCGggGGAGugGggGCGGg -3' miRNA: 3'- -CUCGGCCCCGGUgaCUUUugUa-UGUC- -5' |
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| 6125 | 3' | -52.9 | NC_001826.1 | + | 93277 | 0.69 | 0.835609 |
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Target: 5'- aGGCUGGGGgCGgUGA--AUAUGCAGa -3' miRNA: 3'- cUCGGCCCCgGUgACUuuUGUAUGUC- -5' |
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| 6125 | 3' | -52.9 | NC_001826.1 | + | 19264 | 1.1 | 0.003509 |
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Target: 5'- aGAGCCGGGGCCACUGAAAACAUACAGa -3' miRNA: 3'- -CUCGGCCCCGGUGACUUUUGUAUGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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