miRNA: 3'- gUCagaCGGUGGAGuuUGCACCagag -5'
miRNA: 3'- gUCAGAcGGUGGAGUUUGc-AC-CAGAg -5'
miRNA: 3'- gUCAGACGGUGgAGU---------------UUGCACCagag -5'
miRNA: 3'- gucAGACGGUGGAGUUUgcaCCAG-AG- -5'
miRNA: 3'- -GUCAGACGGUGGAGUUUGCACCAGAG- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6126 | 3' | -53.2 | NC_001826.1 | + | 117831 | 0.66 | 0.948867 |
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Target: 5'- gAGgggGCCGCCUCucGCGUGGg--- -3' miRNA: 3'- gUCagaCGGUGGAGuuUGCACCagag -5' |
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| 6126 | 3' | -53.2 | NC_001826.1 | + | 64058 | 0.67 | 0.917767 |
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Target: 5'- uGGUCU-CCGCCUCAAACacUGaGUCUg -3' miRNA: 3'- gUCAGAcGGUGGAGUUUGc-AC-CAGAg -5' |
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| 6126 | 3' | -53.2 | NC_001826.1 | + | 20082 | 0.67 | 0.907898 |
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Target: 5'- aGGUCUGCCACgUCAcaaguguaccagccaAAUGUGGa--- -3' miRNA: 3'- gUCAGACGGUGgAGU---------------UUGCACCagag -5' |
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| 6126 | 3' | -53.2 | NC_001826.1 | + | 2350 | 0.73 | 0.616667 |
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Target: 5'- --uUCUGCCACCUCAGGaagGGUCaUCa -3' miRNA: 3'- gucAGACGGUGGAGUUUgcaCCAG-AG- -5' |
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| 6126 | 3' | -53.2 | NC_001826.1 | + | 21673 | 1.09 | 0.003859 |
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Target: 5'- cCAGUCUGCCACCUCAAACGUGGUCUCc -3' miRNA: 3'- -GUCAGACGGUGGAGUUUGCACCAGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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