Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6127 | 5' | -53.8 | NC_001826.1 | + | 64153 | 0.66 | 0.930696 |
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Target: 5'- cCCGCCUUgauUCUACCCuaugauuauGUGAG-Ca -3' miRNA: 3'- aGGCGGGAauuAGAUGGGu--------CGCUCaG- -5' |
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| 6127 | 5' | -53.8 | NC_001826.1 | + | 38177 | 0.68 | 0.864705 |
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Target: 5'- uUCCGCCCcag--CUcguCCaGGCGAGUCu -3' miRNA: 3'- -AGGCGGGaauuaGAu--GGgUCGCUCAG- -5' |
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| 6127 | 5' | -53.8 | NC_001826.1 | + | 19678 | 0.68 | 0.856786 |
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Target: 5'- gUCCGCCUggAAUCUGaacggaaugucCCgCAGCG-GUCc -3' miRNA: 3'- -AGGCGGGaaUUAGAU-----------GG-GUCGCuCAG- -5' |
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| 6127 | 5' | -53.8 | NC_001826.1 | + | 119260 | 0.69 | 0.822999 |
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Target: 5'- gCCGCUgUUGGUgccugcCUGCCCAG-GGGUCc -3' miRNA: 3'- aGGCGGgAAUUA------GAUGGGUCgCUCAG- -5' |
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| 6127 | 5' | -53.8 | NC_001826.1 | + | 29087 | 0.7 | 0.776646 |
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Target: 5'- gCUGgCCUgag-CUACCCAcacGCGGGUCa -3' miRNA: 3'- aGGCgGGAauuaGAUGGGU---CGCUCAG- -5' |
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| 6127 | 5' | -53.8 | NC_001826.1 | + | 23674 | 1.1 | 0.002778 |
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Target: 5'- gUCCGCCCUUAAUCUACCCAGCGAGUCu -3' miRNA: 3'- -AGGCGGGAAUUAGAUGGGUCGCUCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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