Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6128 | 3' | -53.7 | NC_001826.1 | + | 28898 | 0.66 | 0.930244 |
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Target: 5'- --aGCUGGUGGacaauaGGGgcUCCAAgGGGGCa -3' miRNA: 3'- gacCGACCACCg-----UCU--AGGUUgUCUUG- -5' |
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| 6128 | 3' | -53.7 | NC_001826.1 | + | 909 | 0.66 | 0.924704 |
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Target: 5'- aUGGUaGaGUGGCAGG-CCAACAuAGCc -3' miRNA: 3'- gACCGaC-CACCGUCUaGGUUGUcUUG- -5' |
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| 6128 | 3' | -53.7 | NC_001826.1 | + | 92297 | 0.66 | 0.906514 |
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Target: 5'- gUGGCUGGUGG-GGGUCacaaAACA-AACa -3' miRNA: 3'- gACCGACCACCgUCUAGg---UUGUcUUG- -5' |
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| 6128 | 3' | -53.7 | NC_001826.1 | + | 28630 | 0.66 | 0.906514 |
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Target: 5'- -aGGCUGGaguggaUGGCGGGgcgcaagCUGGCAGAGa -3' miRNA: 3'- gaCCGACC------ACCGUCUa------GGUUGUCUUg -5' |
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| 6128 | 3' | -53.7 | NC_001826.1 | + | 31980 | 0.67 | 0.899933 |
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Target: 5'- cCUGuGUUGGaucgGGCGGggCCGguuggucgaGCAGGACa -3' miRNA: 3'- -GAC-CGACCa---CCGUCuaGGU---------UGUCUUG- -5' |
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| 6128 | 3' | -53.7 | NC_001826.1 | + | 91980 | 0.67 | 0.871108 |
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Target: 5'- gUGGUaaaGGUGGCAGAUuCCugguACAGGc- -3' miRNA: 3'- gACCGa--CCACCGUCUA-GGu---UGUCUug -5' |
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| 6128 | 3' | -53.7 | NC_001826.1 | + | 44232 | 0.68 | 0.855266 |
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Target: 5'- --uGCUGGUGGCGGGggaaccUCCAagGCGGGu- -3' miRNA: 3'- gacCGACCACCGUCU------AGGU--UGUCUug -5' |
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| 6128 | 3' | -53.7 | NC_001826.1 | + | 6572 | 0.68 | 0.838538 |
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Target: 5'- uUGGUugUGG-GGCAGAUUCuuCAGAGa -3' miRNA: 3'- gACCG--ACCaCCGUCUAGGuuGUCUUg -5' |
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| 6128 | 3' | -53.7 | NC_001826.1 | + | 59436 | 0.69 | 0.793278 |
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Target: 5'- -aGGCUG--GGUAGAUCCAGgAGAGa -3' miRNA: 3'- gaCCGACcaCCGUCUAGGUUgUCUUg -5' |
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| 6128 | 3' | -53.7 | NC_001826.1 | + | 25267 | 1.09 | 0.002834 |
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Target: 5'- uCUGGCUGGUGGCAGAUCCAACAGAACu -3' miRNA: 3'- -GACCGACCACCGUCUAGGUUGUCUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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