Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6128 | 5' | -52.3 | NC_001826.1 | + | 23816 | 0.66 | 0.953486 |
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Target: 5'- aAGaCCAUAgUGG-UGCCACCAGGa- -3' miRNA: 3'- cUCaGGUAUgACUgACGGUGGUCUau -5' |
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| 6128 | 5' | -52.3 | NC_001826.1 | + | 29464 | 0.66 | 0.94453 |
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Target: 5'- aGAGUgaCCAUA--GACUGCCACCAc--- -3' miRNA: 3'- -CUCA--GGUAUgaCUGACGGUGGUcuau -5' |
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| 6128 | 5' | -52.3 | NC_001826.1 | + | 12984 | 0.67 | 0.934514 |
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Target: 5'- ---aCCA-ACUGGCUGCCACCGu--- -3' miRNA: 3'- cucaGGUaUGACUGACGGUGGUcuau -5' |
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| 6128 | 5' | -52.3 | NC_001826.1 | + | 4376 | 0.68 | 0.902771 |
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Target: 5'- cGAGUCCuuggcaaaggcgggcGCUGAgguCUGCCcCCAGAUGu -3' miRNA: 3'- -CUCAGGua-------------UGACU---GACGGuGGUCUAU- -5' |
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| 6128 | 5' | -52.3 | NC_001826.1 | + | 114298 | 0.7 | 0.817095 |
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Target: 5'- uAGcCCuggGCUGAUUGCUGCCAGGUu -3' miRNA: 3'- cUCaGGua-UGACUGACGGUGGUCUAu -5' |
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| 6128 | 5' | -52.3 | NC_001826.1 | + | 25302 | 1.07 | 0.005016 |
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Target: 5'- aGAGUCCAUACUGACUGCCACCAGAUAa -3' miRNA: 3'- -CUCAGGUAUGACUGACGGUGGUCUAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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