Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6129 | 3' | -49.6 | NC_001826.1 | + | 30258 | 0.66 | 0.992375 |
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Target: 5'- gAACAcGGCCgauGAUGGAaCGCUgUGUGGg -3' miRNA: 3'- -UUGUaUCGGgu-UUACCU-GUGA-ACGCC- -5' |
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| 6129 | 3' | -49.6 | NC_001826.1 | + | 116971 | 0.67 | 0.984989 |
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Target: 5'- cAGCGUGGCCUggGcguUGGAUGCggGCa- -3' miRNA: 3'- -UUGUAUCGGGuuU---ACCUGUGaaCGcc -5' |
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| 6129 | 3' | -49.6 | NC_001826.1 | + | 13879 | 0.67 | 0.984989 |
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Target: 5'- --gAUAGUCCAGGcUGGGCAUggaaaggaUUGUGGg -3' miRNA: 3'- uugUAUCGGGUUU-ACCUGUG--------AACGCC- -5' |
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| 6129 | 3' | -49.6 | NC_001826.1 | + | 40215 | 0.68 | 0.975898 |
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Target: 5'- ---uUGGCCC-AAUGGACGCacccGUGGa -3' miRNA: 3'- uuguAUCGGGuUUACCUGUGaa--CGCC- -5' |
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| 6129 | 3' | -49.6 | NC_001826.1 | + | 26215 | 0.74 | 0.78101 |
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Target: 5'- aGACAUGGCCUAuc--GAcCGCUUGCGGu -3' miRNA: 3'- -UUGUAUCGGGUuuacCU-GUGAACGCC- -5' |
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| 6129 | 3' | -49.6 | NC_001826.1 | + | 26033 | 1.09 | 0.008558 |
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Target: 5'- aAACAUAGCCCAAAUGGACACUUGCGGu -3' miRNA: 3'- -UUGUAUCGGGUUUACCUGUGAACGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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