Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6129 | 5' | -55.5 | NC_001826.1 | + | 25364 | 0.66 | 0.867305 |
Target: 5'- uGGCUGCAAGgGGUCcu--GuGCCGUGUc -3' miRNA: 3'- -UUGGCGUUCgCCAGcuauC-CGGUACA- -5' |
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6129 | 5' | -55.5 | NC_001826.1 | + | 13800 | 0.67 | 0.780009 |
Target: 5'- uAGCCGCAAGaUGGcauuguugagUCGcugcuucgcGUAGGCCAUGa -3' miRNA: 3'- -UUGGCGUUC-GCC----------AGC---------UAUCCGGUACa -5' |
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6129 | 5' | -55.5 | NC_001826.1 | + | 26181 | 0.68 | 0.750626 |
Target: 5'- uAACCGCAAGU-GUCcauuUGGGCUAUGUu -3' miRNA: 3'- -UUGGCGUUCGcCAGcu--AUCCGGUACA- -5' |
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6129 | 5' | -55.5 | NC_001826.1 | + | 26068 | 1.04 | 0.004116 |
Target: 5'- aAACCGCAAGCGGUCGAUAGGCCAUGUc -3' miRNA: 3'- -UUGGCGUUCGCCAGCUAUCCGGUACA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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