Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 613 | 3' | -61.5 | AC_000017.1 | + | 15540 | 0.66 | 0.273198 |
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Target: 5'- aCGGGgUCUUGCAucauGUCGGGCaacgACCAGu -3' miRNA: 3'- -GCUCgAGAGCGU----CGGCCCGg---UGGUCu -5' |
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| 613 | 3' | -61.5 | AC_000017.1 | + | 12553 | 0.68 | 0.200163 |
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Target: 5'- gGGGUUUgCGCGcGCCGGGaCCACCGc- -3' miRNA: 3'- gCUCGAGaGCGU-CGGCCC-GGUGGUcu -5' |
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| 613 | 3' | -61.5 | AC_000017.1 | + | 16348 | 0.68 | 0.198048 |
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Target: 5'- aCGAGCggccgccgcaGCAGCCGcGGCCAUUAGu -3' miRNA: 3'- -GCUCGagag------CGUCGGC-CCGGUGGUCu -5' |
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| 613 | 3' | -61.5 | AC_000017.1 | + | 8403 | 0.69 | 0.191309 |
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Target: 5'- gGAGCUCcugcagguuuaccUCGCauAGCCGGGUCAgggcgcgggcuagguCCAGGu -3' miRNA: 3'- gCUCGAG-------------AGCG--UCGGCCCGGU---------------GGUCU- -5' |
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| 613 | 3' | -61.5 | AC_000017.1 | + | 913 | 0.69 | 0.170437 |
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Target: 5'- -aGGCUCUCuGCuccggcuGCuCGGGCUGCCGGGa -3' miRNA: 3'- gcUCGAGAG-CGu------CG-GCCCGGUGGUCU- -5' |
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| 613 | 3' | -61.5 | AC_000017.1 | + | 10303 | 1.08 | 0.000158 |
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Target: 5'- cCGAGCUCUCGCAGCCGGGCCACCAGAc -3' miRNA: 3'- -GCUCGAGAGCGUCGGCCCGGUGGUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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