Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6130 | 3' | -60.6 | NC_001826.1 | + | 3954 | 0.66 | 0.622904 |
Target: 5'- uCUCCUGACCaaACCCCCaGU-GaGUGCUg -3' miRNA: 3'- -GAGGGCUGG--UGGGGGaCAgCcUACGA- -5' |
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6130 | 3' | -60.6 | NC_001826.1 | + | 119050 | 0.7 | 0.397055 |
Target: 5'- -gCCCG-CCGcgcCCCCCUGcCGGggGCUg -3' miRNA: 3'- gaGGGCuGGU---GGGGGACaGCCuaCGA- -5' |
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6130 | 3' | -60.6 | NC_001826.1 | + | 118454 | 0.71 | 0.349081 |
Target: 5'- -cCCCGACCccaGCCCCC--UCGGcgGCUc -3' miRNA: 3'- gaGGGCUGG---UGGGGGacAGCCuaCGA- -5' |
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6130 | 3' | -60.6 | NC_001826.1 | + | 118754 | 0.72 | 0.312366 |
Target: 5'- -cCCCGGgcCCGCCCCCggggGUCGGggGUc -3' miRNA: 3'- gaGGGCU--GGUGGGGGa---CAGCCuaCGa -5' |
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6130 | 3' | -60.6 | NC_001826.1 | + | 26361 | 1.07 | 0.001267 |
Target: 5'- cCUCCCGACCACCCCCUGUCGGAUGCUu -3' miRNA: 3'- -GAGGGCUGGUGGGGGACAGCCUACGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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