Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6133 | 5' | -57.9 | NC_001826.1 | + | 118538 | 0.67 | 0.700222 |
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Target: 5'- ---uGGCcCUCGGAGGCCGGAgagGGCc- -3' miRNA: 3'- caauCUGuGGGUCUCCGGCCU---CCGuu -5' |
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| 6133 | 5' | -57.9 | NC_001826.1 | + | 12629 | 0.67 | 0.648283 |
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Target: 5'- -gUAGGCaggcacgguaguGCCCuGAGGCgUGGAGGCu- -3' miRNA: 3'- caAUCUG------------UGGGuCUCCG-GCCUCCGuu -5' |
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| 6133 | 5' | -57.9 | NC_001826.1 | + | 118904 | 0.69 | 0.564895 |
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Target: 5'- --gGGGCcggguuCgCCGGGGGUCGGGGGCGGg -3' miRNA: 3'- caaUCUGu-----G-GGUCUCCGGCCUCCGUU- -5' |
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| 6133 | 5' | -57.9 | NC_001826.1 | + | 118751 | 0.69 | 0.544394 |
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Target: 5'- --cGGGCccgccCCCGGGGGUCGGGGGUc- -3' miRNA: 3'- caaUCUGu----GGGUCUCCGGCCUCCGuu -5' |
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| 6133 | 5' | -57.9 | NC_001826.1 | + | 11004 | 0.69 | 0.544394 |
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Target: 5'- --gGGACGCCgUAGAGGCaguuuGGGGGCu- -3' miRNA: 3'- caaUCUGUGG-GUCUCCGg----CCUCCGuu -5' |
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| 6133 | 5' | -57.9 | NC_001826.1 | + | 118334 | 0.7 | 0.494324 |
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Target: 5'- uGUUGGuGCcugccuGCCCAGGGGUgGGGGGUAGg -3' miRNA: 3'- -CAAUC-UG------UGGGUCUCCGgCCUCCGUU- -5' |
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| 6133 | 5' | -57.9 | NC_001826.1 | + | 119039 | 0.76 | 0.211719 |
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Target: 5'- -cUGGAgACCCGG-GGCUGGGGGCGc -3' miRNA: 3'- caAUCUgUGGGUCuCCGGCCUCCGUu -5' |
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| 6133 | 5' | -57.9 | NC_001826.1 | + | 33343 | 1.05 | 0.00211 |
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Target: 5'- gGUUAGACACCCAGAGGCCGGAGGCAAc -3' miRNA: 3'- -CAAUCUGUGGGUCUCCGGCCUCCGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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