Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6135 | 3' | -57.1 | NC_001826.1 | + | 4980 | 0.67 | 0.783542 |
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Target: 5'- uACGUGGUcuaucuguggaucucGGuUCAAGUCCGAGUcCUggCCa -3' miRNA: 3'- -UGCACCA---------------CC-GGUUCAGGCUCGaGA--GG- -5' |
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| 6135 | 3' | -57.1 | NC_001826.1 | + | 7251 | 0.67 | 0.760907 |
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Target: 5'- gAUGUGGauaggaaGGCCAuggCUGAcGCUCUCCc -3' miRNA: 3'- -UGCACCa------CCGGUucaGGCU-CGAGAGG- -5' |
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| 6135 | 3' | -57.1 | NC_001826.1 | + | 95851 | 0.68 | 0.705728 |
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Target: 5'- uUGUGGUGGaCGuggguauaagaugauAGgggCUGGGCUCUCCc -3' miRNA: 3'- uGCACCACCgGU---------------UCa--GGCUCGAGAGG- -5' |
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| 6135 | 3' | -57.1 | NC_001826.1 | + | 90740 | 0.69 | 0.681378 |
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Target: 5'- -aGUGGUGGCCc-GUCCcaAGaCUCUCUc -3' miRNA: 3'- ugCACCACCGGuuCAGGc-UC-GAGAGG- -5' |
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| 6135 | 3' | -57.1 | NC_001826.1 | + | 30956 | 0.7 | 0.588926 |
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Target: 5'- gGCGUGGggGGUgGAGgcucCUGAGCUaaaCUCCa -3' miRNA: 3'- -UGCACCa-CCGgUUCa---GGCUCGA---GAGG- -5' |
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| 6135 | 3' | -57.1 | NC_001826.1 | + | 52430 | 0.7 | 0.568535 |
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Target: 5'- uCGUgGGUGGCaGGGUCCuuauAGCUCUUCa -3' miRNA: 3'- uGCA-CCACCGgUUCAGGc---UCGAGAGG- -5' |
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| 6135 | 3' | -57.1 | NC_001826.1 | + | 167 | 0.74 | 0.389688 |
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Target: 5'- --cUGGUGGUucucgguuCAAGUCCGAGCUCUg- -3' miRNA: 3'- ugcACCACCG--------GUUCAGGCUCGAGAgg -5' |
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| 6135 | 3' | -57.1 | NC_001826.1 | + | 41752 | 1.12 | 0.001103 |
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Target: 5'- gACGUGGUGGCCAAGUCCGAGCUCUCCa -3' miRNA: 3'- -UGCACCACCGGUUCAGGCUCGAGAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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