Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6135 | 5' | -59.7 | NC_001826.1 | + | 113499 | 0.66 | 0.632365 |
Target: 5'- -cGCUGGGCaUCGCCau--GCCCAUCa -3' miRNA: 3'- aaCGACUCG-GGUGGguacCGGGUAGa -5' |
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6135 | 5' | -59.7 | NC_001826.1 | + | 26189 | 0.67 | 0.590811 |
Target: 5'- -aGCUGuGCUCACauCCuauUGGCCUGUCa -3' miRNA: 3'- aaCGACuCGGGUG--GGu--ACCGGGUAGa -5' |
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6135 | 5' | -59.7 | NC_001826.1 | + | 29088 | 0.67 | 0.590811 |
Target: 5'- -gGCUG-GCCUgagcuACCCAcacgcgggucagUGGCCUAUCa -3' miRNA: 3'- aaCGACuCGGG-----UGGGU------------ACCGGGUAGa -5' |
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6135 | 5' | -59.7 | NC_001826.1 | + | 41787 | 1.05 | 0.001258 |
Target: 5'- gUUGCUGAGCCCACCCAUGGCCCAUCUa -3' miRNA: 3'- -AACGACUCGGGUGGGUACCGGGUAGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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