Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6144 | 3' | -58.5 | NC_001826.1 | + | 115671 | 0.66 | 0.754683 |
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Target: 5'- uCAGAuGCuGCAggGAcuucuguGGAGaGGCUGGCGu -3' miRNA: 3'- -GUCU-CG-CGUagCUu------CCUC-CCGACCGC- -5' |
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| 6144 | 3' | -58.5 | NC_001826.1 | + | 70856 | 0.66 | 0.7352 |
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Target: 5'- aCGGGGUGCAUgGGcgcuguagucuGGGGGGauGCUGGUu -3' miRNA: 3'- -GUCUCGCGUAgCU-----------UCCUCC--CGACCGc -5' |
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| 6144 | 3' | -58.5 | NC_001826.1 | + | 104648 | 0.66 | 0.714356 |
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Target: 5'- uGGAGCGCggCGucuuuuAGGAGGcauggcuGCUGGUu -3' miRNA: 3'- gUCUCGCGuaGCu-----UCCUCC-------CGACCGc -5' |
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| 6144 | 3' | -58.5 | NC_001826.1 | + | 118237 | 0.67 | 0.705322 |
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Target: 5'- gGGGGUGCuggggggUGGAGG-GGGCUGGa- -3' miRNA: 3'- gUCUCGCGua-----GCUUCCuCCCGACCgc -5' |
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| 6144 | 3' | -58.5 | NC_001826.1 | + | 70324 | 0.67 | 0.695225 |
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Target: 5'- aGGGGUGaguUCGAcGGuAGGGgaGGCGg -3' miRNA: 3'- gUCUCGCgu-AGCUuCC-UCCCgaCCGC- -5' |
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| 6144 | 3' | -58.5 | NC_001826.1 | + | 118815 | 0.67 | 0.674881 |
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Target: 5'- -cGGGCcCGg-GGAGGGGGGCcgGGCGg -3' miRNA: 3'- guCUCGcGUagCUUCCUCCCGa-CCGC- -5' |
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| 6144 | 3' | -58.5 | NC_001826.1 | + | 118590 | 0.67 | 0.664651 |
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Target: 5'- gAGGGgGCugggGUCGggGGAGuGGg-GGCGg -3' miRNA: 3'- gUCUCgCG----UAGCuuCCUC-CCgaCCGC- -5' |
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| 6144 | 3' | -58.5 | NC_001826.1 | + | 23911 | 0.67 | 0.661577 |
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Target: 5'- aGGGGCuCAUCGGgcuuaaaguccaccAGGGGGGUgacUGGCa -3' miRNA: 3'- gUCUCGcGUAGCU--------------UCCUCCCG---ACCGc -5' |
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| 6144 | 3' | -58.5 | NC_001826.1 | + | 98006 | 1.07 | 0.00153 |
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Target: 5'- cCAGAGCGCAUCGAAGGAGGGCUGGCGc -3' miRNA: 3'- -GUCUCGCGUAGCUUCCUCCCGACCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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